AUTHOR=Martinho Filipe , Pina Beatriz , Nunes Margarida , Vasconcelos Rita P. , Fonseca Vanessa F. , Crespo Daniel , Primo Ana Lígia , Vaz Ana , Pardal Miguel A. , Gillanders Bronwyn M. , Tanner Susanne E. , Reis-Santos Patrick 

TITLE=Water and Otolith Chemistry: Implications for Discerning Estuarine Nursery Habitat Use of a Juvenile Flatfish

JOURNAL=Frontiers in Marine Science

VOLUME=7

YEAR=2020

URL=https://www.frontiersin.org/journals/marine-science/articles/10.3389/fmars.2020.00347

DOI=10.3389/fmars.2020.00347

ISSN=2296-7745

ABSTRACT=<p>Variations in otolith elemental composition are widely used to reconstruct fish movements. However, reconstructing habitat use and environmental histories of fishes within estuaries is still a major challenge due to the dynamic nature of these coastal environments. In this study, we performed a laboratory experiment to investigate the effects of variations in salinity (three levels; 5, 18, 30) and temperature (two levels; 16, 21°C) on the otolith elemental composition (Mg:Ca, Mn:Ca, Sr:Ca, Ba:Ca) of juvenile Senegalese sole <italic>Solea senegalensis.</italic> Temperature and salinity treatments mirrored the natural conditions of the estuarine habitats occupied by juvenile Senegalese sole, thereby providing information on the applicability of otolith microchemistry to reconstruct habitat use patterns within estuarine nurseries, where individual fish move across complex salinity and temperature gradients. While Sr:Ca and Ba:Ca in otoliths were both positively related to salinity, no temperature effect was observed. Partition coefficients, proxies for element incorporation rates increased with increasing salinity for Sr (D<sub>Sr</sub>) and Ba (D<sub>Ba</sub>). In contrast, salinity and temperature had little influence on otolith Mn:Ca and Mg:Ca, supporting physiological control on the incorporation of these elements. Our results are a stepping stone for the interpretation of otolith chemical profiles for fish collected in their natural habitats and contribute to better understanding the processes involved in otolith element incorporation.</p>