AUTHOR=Guo Baoying , Xu Zhongtian , Yan Xiaojun , Buttino Isabella , Li Jiji , Zhou Chao , Qi Pengzhi 

TITLE=Novel ABCB1 and ABCC Transporters Are Involved in the Detoxification of Benzo(α)pyrene in Thick Shell Mussel, Mytilus coruscus

JOURNAL=Frontiers in Marine Science

VOLUME=7

YEAR=2020

URL=https://www.frontiersin.org/journals/marine-science/articles/10.3389/fmars.2020.00119

DOI=10.3389/fmars.2020.00119

ISSN=2296-7745

ABSTRACT=<p>ATP-binding cassette (ABC) transporters play an essential role in xenobiotic resistance through transporting xenobiotics into or out of cells. However, their functional role in mollusk's defense against polycyclic aromatic hydrocarbons (PAHs) remains unclear. In the present study, novel <italic>Mco</italic>ABCB1 and <italic>Mco</italic>ABCC transporters were identified in the gills of <italic>Mytilus coruscus</italic>, and their benzo(α)pyrene (Bap) detoxification function was determined by qRT-PCR and efflux assays. <italic>Mco</italic>ABCB1 and <italic>Mco</italic>ABCC genes were constitutively expressed in all tissue types, suggesting the primary physiological function of these transporters. Bap, a chemical stress agent, could induce the mRNA expression of <italic>Mco</italic>ABCB1 and <italic>Mco</italic>ABCC in gills. However, at high concentration, Bap inhibited their expression levels at the late stage. Besides, the efflux activities in <italic>M. coruscus</italic> gills showed a dose-dependent elevation in a Bap pre-exposure group, compared with those in a reversin 205 or MK571 treatment alone group. These results demonstrated the presence of ABCB1 and ABCC transporters in <italic>Mytilus coruscus</italic>, and their involvement in cellular Bap detoxification.</p>