Thaiane Verly ¹ Federico Gastón Fiad ² Ana Laura Carbajal-de-la-Fuente ^1* Sebastián Pita ³ Romina Valeria Piccinali ⁴ Patricia Alejandra Lobbia ⁵ Paz Sánchez Casaccia ¹ Gladys Antonieta Rojas-de-Arias ⁶ María José Cavallo ⁷ Gisel V Gigena ² Claudia S Rodríguez ² María C Vega- Gómez ⁶ Miriam Rolón ⁶ Julieta Nattero ^4*

• ¹ National Center for Diagnosis and Research in Endemo - Epidemics, Administración Nacional de Laboratorios e Institutos de Salud (ANLIS), Buenos Aires, Buenos Aires, Argentina
• ² Instituto de Investigaciones Biológicas y Tecnológicas (IIByT), Códoba, Argentina
• ³ Sección Genética Evolutiva, Departamento de Biología Animal, Instituto de Biología, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay
• ⁴ Institute of Ecology, Genetics and Evolution of Buenos Aires, University of Buenos Aires, Ciudad autónoma de Buenos Aires, Argentina
• ⁵ Unidad Operativa de Vectores y Ambiente (UnOVE), CeNDIE, Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos Malbrán, Santa Maria de Punilla, Argentina
• ⁶ Center for the Development of Scientific Research (CEDIC), Asuncion, Paraguay
• ⁷ Centro Regional de Energía y Ambiente para el Desarrollo Sustentable (CREAS), Catamarca, Argentina

Triatoma garciabesi is a vector of Trypanosoma cruzi, the causative agent of Chagas disease, is found across northwest and central Argentina, southern Bolivia, and western Paraguay. Frequently invades rural houses during the warm seasons and is common in peridomestic and wild environments. Recently, the existence of two lineages has been demonstrated based on variation in cytochrome c oxidase I gene (coI). These lineages occur across the species distribution range and coincide with different ecological regions. Here we aim to examine how phenotypic variation in flight-related traits is structured; determine the association between these traits and geographic distance and identify the climatic, geographic, and/or vegetation cover variables that best explain the morphometric variation in flight-related traits.A total of 198 males of T. garciabesi from 24 populations in Argentina and Paraguay were included in this study, covering almost the entire T. garciabesi distribution range. Size and shape components of the forewing, membranous and stiff portions of the forewing and head were measured using a landmark-based methodology. Our study documents that the size component of the membranous and stiff portions showed significant variation across the species range. Although forewing and head shape did not show significant differences in Procrustes distances across all pairs of populations, the membranous and stiff portions did.There is a strong and consistent association between shape and geographic distances at all levels of comparison (species and lineage ranges). The size and shape components, geographic, climatic and/or vegetation indexes explained covariation in all flight-related traits. Triatoma garciabesi appears to be a species sensitive to vegetation cover and landscape features. This study provides evidence for this by showing clear variation in flight-related traits across the species and lineage distribution range, as well as indications of isolation by distance and variation in flight-related traits according to climate, geography, and vegetation cover.