Corrigendum: Adeno-Associated Virus as an Effective Malaria Booster Vaccine following Adenovirus Priming

Yenni Yusuf ^1,2 Tatsuya Yoshii ¹ Mitsuhiro Iyori ¹ Kunitaka Yoshida ¹ Hiroaki Mizukami ³ Shinya Fukumoto ⁴ Asrar Alam ¹ Talha Bin Emran ¹ Fitri Amelia ¹ Ashekul Islam ¹ Hiromu Otsuka ¹ Eizo Takashima ⁵ Takafumi Tsuboi ⁵ Shigeto Yoshida ^1*

• ¹ Kanazawa University, Kanazawa, Japan
• ² Hasanuddin University, Makassar, South Sulawesi, Indonesia
• ³ Division of Gene Therapy, Jichi Medical University, Tochigi, Tochigi, Japan
• ⁴ Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan
• ⁵ Ehime University, Matsuyama, Ehime, Japan

Corrigendum on: figure 3 of the article. In the published article, there was an error in Figure 3 as published. In the figure 3C, the figure of the non-permeabilized AdHu5-PfCSP is exactly similar with that of non-permeabilized AAV1-PfCSP-G(+). The corrected Figure 3 and its caption **Fig 3. Functional activity of AAV1-PfCSP-G(+). (A) Constructs of AAV1-PfCSP-G(+) and AdHu5-PfCSP-G(+). Expression of the pcsp gene cassette in AAV1 and AdHu5 was driven by a CMV promoter and CAG promoter, respectively. G, VSV-G. (B) Expression of PfCSP in HEK293T cells transduced with AdHu5-PfCSP (lane 1, MOI = 3), AAV1-PfCSP-G(–) (lane 2, MOI = 105 ), or AAV1-PfCSP-G(+) (lane 3, MOI = 105 ), as assessed by immunoblotting with mAb 2A10 at 48 h post-transduction. (C) Localization of PfCSP expression in HEK293T cells transduced with AAV1-PfCSP-G(+) (MOI = 105) and AdHu5-PfCSP (MOI = 10), as determined by IFA conducted as described in Figure 2C. (D) Anti-PfCSP IgG antibody responses. Groups of BALB/c mice (n = 10) were immunized with the indicated regimen at a 6-week interval. At 4 weeks post-boost, serum samples were collected from each mouse, and their anti-PfCSP IgG titers were determined by ELISA. AdHu5-PfCSP, AAV1-PfCSP-G(–), and AAV1-PfCSP (G+) are shown as AdHu5, AAV1-G(–), and AAV1-G(+), respectively. Bars and error bars indicate the means and SD of the values, respectively. Between-group differences were assessed with a Mann–Whitney U-test (*p < 0.05). appear below.