Corrigendum: Mannose-modified erythrocyte membrane-encapsulated chitovanic nanoparticles as a DNA vaccine carrier against reticuloendothelial tissue hyperplasia virus

A Corrigendum on
Mannose-modified erythrocyte membrane-encapsulated chitovanic nanoparticles as a DNA vaccine carrier against reticuloendothelial tissue hyperplasia virus

By Feng Y, Tang F, Li S, Wu D, Liu Q, Li H, Zhang X, Liu Z, Zhang L and Feng H (2023) Front. Immunol. 13:1066268. doi: 10.3389/fimmu.2022.1066268

In this published article, there was an error in Figure 3 as published. The incorrect image for group PBS, CS-gp90@M, and CS-gp90@M-M in Figure 3B was uploaded by mistake. The corrected Figure 3 and its caption appears below.

Figure 3

Figure 3. CS-gp90@M-M uptake by macrophages. (A) CLSM image of DiD-stained CS-gp90@M and CS-gp90@M-M nanoparticles up take by macrophages in vitro. Macrophage solution (8 × 10⁴ cells/mL) was added to a 24-well plate, and 50 μL of DiD-stained CS-gp90@M and CS-gp90@M-M (200 μg/mL) nanoparticles were added and incubated for 8 h. Then, the cells were stained with DAPI dye and washed twice for 20 min. The cells were mounted using glycerol (90%) and observed by CLSM. Blue fluorescence represents DAPI-stained nuclei and red fluorescence represents DID-stained CS-gp90@M and CS-gp90@M-M nanoparticles. (B) The uptake of CS-gp90@M-M nanoparticles by macrophages using an IVIS instrument. Macrophages were added to a 6-well plate and cultured for 24 h. DiD-stained CS-gp90@M (50 μL) or CS-gp90@M-M (200 μg/mL) nanoparticles were added to macrophages (5 × 10⁵) in a Petri dish for 8 h. The cells were collected and the intracellular fluorescence intensity of DID was determined using the IVIS instrument. The image shows the fluorescence intensity of the cellular uptake of CS-gp90@M and CS-gp90@M-M nanoparticles by macrophages. (C) The average radiant efficiency of the macrophage uptake of nanoparticles.

In this published article, there was an error in the legend for Figure 6 as published. The incorrect body and organ image for group CS-gp90@M-M at 168h in Figure 6A and Figure 6C was incorrectly uploaded. The corrected Figure 6 and its caption appears below.

Figure 6

Figure 6. Release and biodistribution of CS-gp90@M-M NPs in vivo. (A, B) In vivo fluorescence images of chicks and the attenuation of the fluorescent dyes over time (C–F) Direct imaging of excised organs. Live animal imaging of chicks. The vaccine formulation was stained using a Cy5.5 fluorescent dye and chicks were immunized with CS-gp90@M-M and CS-gp90@M NPs. Live-animal imaging and fluorescence intensity in chicks at 24, 48, 72, and 168 h after injection was determined using an in vivo optical imaging system (A, B). Direct imaging and fluorescence intensity of the bursa, spleens, and livers of the injected chicks was determined at 12, 24, 72, 120, and 168 h after injection (C–F).

In the published article, there was an error in Figure 8 as published. The incorrect use of pathological sections of spleen in the CS-gp90@M group were uploaded in Figure 8. The corrected Figure 8 and its caption appears below.

Figure 8

Figure 8. Analysis of potential in vivo toxicity. H&E staining of the lungs, heart, spleen, liver, and kidneys of vaccinated chicks on day 28 after immunization. Magnification: 100×, scale bars: 100 μm.

The authors apologize for these errors and state that they do not change the scientific conclusions of the article in any way.

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