Longitudinal Analysis of hsa-miR-3163, hsa-miR-124-3p, hsa-miR-548c-3p, and hsa-miR-27a-3p as Prognostic Biomarkers in HIV Patients

Ilker Inanç Balkan^1,2,3Andleeb Shahzadi^4*Haktan Sonmez⁴Burhaneddin Oktan⁴Muhammed ıhtisham Umar⁵Bilgül Mete¹Gunnur Deniz²Fehmi Tabak¹Umut Can Kucuksezer²

• ¹Department of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, Istanbul University Cerrahpasa, Istanbul, Türkiye
• ²Department of Immunology, Aziz Sancar Institute of Experimental Medicine, Istanbul University, Istanbul, Türkiye
• ³Institute of Graduate Studies in Health Sciences,Istanbul university, ıstanbul, Türkiye
• ⁴Department of Medical Pharmacology, Cerrahpasa Medical Faculty, Istanbul University-Cerrahpasa, Istanbul, Türkiye
• ⁵COMSATS University Islamabad, Lahore Campus, Lahore, Punjab, Pakistan

This study investigates the expression patterns and prognostic potential of specific microRNAs (miRNAs) in HIV-positive individuals undergoing antiretroviral therapy (ART). The miRNAs analyzed; miR-124-3p, miR-27a-3p, miR-548ac-3p, and miR-3163 were carefully selected through rigorous bioinformatic analysis as part of a larger project aimed at understanding the molecular mechanisms of HIV pathogenesis and treatment outcomes. The primary goal is to identify miRNAs with significant prognostic value, focusing on prediction of treatment response and monitoring disease progression. While this study does not extend to therapeutic modulation or detailed mechanistic roles of these miRNAs, it provides a strong foundation for future research. By integration of bioinformatics with clinical data, the findings lay the groundwork for using these miRNAs as reliable biomarkers. This research offers key insights to advance personalized HIV management and develop more targeted, effective treatments.