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ORIGINAL RESEARCH article
Front. Immunol.
Sec. Viral Immunology
Volume 16 - 2025 | doi: 10.3389/fimmu.2025.1556591
This article is part of the Research TopicAnimal-borne viral disease: Pathogenesis, Innate immunity, Acquired immunity, and Novel vaccine developmentView all 9 articles
MicroRNA-1985 enhances the redox capability of scallop (Patinopecten yessoensis) in response to poly(I:C) stimulation by targeting MNK1
Provisionally accepted
Linghui Yu1Huiqi Deng2Shaohua Liu2Jianpin Xia2
Hao Zhen Lin2Donghong Yin2
Yaoyao Zhan2*
Yaqing Chang1,2- 1Shenyang Agricultural University, Shenyang, Liaoning, China
- 2Dalian Ocean University, Dalian, China
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To clarify the microRNA (miRNA)-target gene axis is involved in response to pathogen-associated molecular pattern (PAMP)-induced oxidative stress in shellfish, the full-length cDNA of a novel mitogen-activated protein kinase-interacting kinase 1 (MNK1) homolog gene from the scallop Patinopecten yessoensis (PyMNK1) was cloned and characterized. The interaction between miR-1985 and PyMNK1 was verified, and then the responses and possible molecular function of miR-1985, PyMNK1, and miR-1985/PyMNK1 axis to poly(I:C) (a classic virus-related PAMP) stimulation in P. yessoensis were explored and preliminarily dissected. The results indicate: 1) The full-length cDNA of PyMNK1 was 5354 bp, with a high level of sequence conservation across mollusks. 2) MiR-1985 bound to the 3'-UTR of PyMNK1 and negatively regulated the expression of PyMNK1. 3) PyMNK1 may repress the relative expression of superoxide dismutase (SOD) by binding its promoter. 4) Both PyMNK1 silencing and miR-1985 overexpression promoted the expression and enzymatic activity of SOD. 5) The miR-1985/PyMNK1 axis may be involved in the response to poly(I:C) stimulation by elevating the activity of the SOD/catalase axis. To summarize, all observations from this study indicated that P. yessoensis may enhance its redox capability via the miR-1985/PyMNK1/SOD/CAT cascade and thereby alleviate PAMP-induced oxidative stress.
Keywords: miR-1985, mitogen-activated protein kinase-interacting kinase 1, Patinopecten yessoensis, Oxidative Stress, innate immunity
Received: 07 Jan 2025; Accepted: 15 Apr 2025.
Copyright: © 2025 Yu, Deng, Liu, Xia, Lin, Yin, Zhan and Chang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Yaoyao Zhan, Dalian Ocean University, Dalian, China
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