AUTHOR=Moleirinho Beatriz , Paulo-Pedro Margarida , Martins Nicole C. , Jelagat Emily , Conti Eller , Velho Tiago R. , Abecasis Miguel , Anjos Rui , Almeida Afonso R. M. , Sousa Ana E. TITLE=A backbone-based flow cytometry approach to decipher regulatory T cell trajectories in the human thymus JOURNAL=Frontiers in Immunology VOLUME=16 YEAR=2025 URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2025.1553535 DOI=10.3389/fimmu.2025.1553535 ISSN=1664-3224