The nature of the post-translational modifications of the autoantigen LL37 influences the autoreactive T-helper cell phenotype in psoriasis

Roberto Lande ¹ Anna Mennella ¹ Raffaella Palazzo ¹ Rebecca Favaro ² Paola Facheris ³ Flavia Mancini ¹ Giuseppe Ocone ¹ Elisabetta Botti ⁴ Mario Falchi ¹ Immaolata Pietraforte ¹ Curdin Conrad ⁵ Luca Bianchi ⁶ Antonio Costanzo ² Loredana Frasca ^1*

• ¹ National Institute of Health (ISS), Rome, Lazio, Italy
• ² Centro di Ricerca Humanitas, Skin Pathology Lab, Rozzano, Milan, Italy,, Rozzano, Milan, Italy
• ³ Humanitas University, Rozzano, Italy
• ⁴ Policlinico Tor Vergata, Rome, Lazio, Italy
• ⁵ Centre Hospitalier Universitaire Vaudois (CHUV), Lausanne, Vaud, Switzerland
• ⁶ University of Rome Tor Vergata, Roma, Lazio, Italy

Psoriasis is a chronic skin disease evolving to psoriatic arthritis (PsA) in 30% of cases.LL37 is a psoriasis T-cell autoantigen and, in complex with self-DNA/RNA, a trigger of type I interferon (IFN-I) and pro-inflammatory factors in dendritic cells. LL37 can undergo irreversible post-translational modifications (PTMs), namely citrullination and carbamylation, which are linked to a neutrophil-dominated inflammation. Notably, in PsA, carbamylated and citrullinated LL37 (carb-LL37 and cit-LL37) become antibody targets.Here, we analyze the presence of, and the T-cell and antibody reactivity to, cit-LL37 and carb-LL37, to address occurrence and significance of these PTMs in psoriasis. The presence of modified LL37 in skin biopsies was assessed by laser scanner confocal microscopy (LSCM); T-cell responses to modified LL37 was assessed by Ki67 assay and intracellular cytokines staining using flow-cytometry; serum autoantibodies to the same antigens were tested by ELISA. The results show that: native and modified LL37 (both carb-LL37 and cit-LL37) are detectable in psoriatic skin, but not in healthy donors (HD) skin, where they colocalize with neutrophil infiltrates and neutrophil extracellular trap formation (NETosis).Psoriatic T-cells and antibodies recognize native LL37, cit-LL37 and carb-LL37, but only CD4-T-cell responses to native LL37 and carb-LL37 correlate with psoriasis area severity 2 index (PASI), whereas CD8-T-cell responses to the same peptides correlate with PASI in the HLA-Cw6*02-positive subgroup. CD4-T-cells specific for modified LL37 express heterogeneous T-helper (Th) phenotypes: native/carb-LL37-specific T-cells mainly manifest a Th1/Th17-like phenotype, whereas cit-LL37-specific T-cells resemble Thfollicular (Thf)-like cells. In vitro T-cell polarization experiments suggest that distinct proinflammatory effects of LL37 and modified LL37, in complex with self-nucleic acids, may concur to these phenomena. This is the first evidence in psoriasis that PTMs of an autoantigen with innate immune cell stimulatory ability dictates autoreactive Th-cellpolarization. This data, obtained using LL37 as a model autoantigen, indicate that citrullination and carbamylation pathways may play a role in the psoriasis course generating epitopes to which immunological tolerance does not exist, and potentially concur to PsA development.