Disruption of the moonlighting function of CTF18 in a patient with T-lymphopenia

Robert Sertori ¹ Billy Truong ¹ Manoj K Singh ¹ Susan Shinton ¹ Rachael Price ¹ Andrew Sharo ² Paulameena Shultes ² Uma Sunderam ³ Sadhna Rana ³ Rajgopal Srinivasan ³ Sutapa Datta ³ Joan Font-Burgada ¹ Steven E Brenner ² Jennifer M Puck ² David L. Wiest ^1*

• ¹ Immune Cell Development and Host Defense Program, Fox Chase Cancer Center, Philadelphia, PA, United States
• ² University of California, Berkeley, Berkeley, California, United States
• ³ Tata Consultancy Services (India), Kolkata, West Bengal, India

Newborn screening for immunodeficiency has led to the identification of numerous cases for which the causal etiology is unknown. Here we report the diagnosis of T lymphopenia of unknown etiology in a male proband. Whole exome sequencing (WES) nominated as the top candidate variants, missense mutations in CHTF18 that were inherited in an autosomal recessive manner. CTF18, encoded by the CHTF18 gene, is a component of a secondary clamp loader, which is primarily thought to function by promoting DNA replication. We determined that the patient's variants in CHTF18 (CTF18 R751W and E851Q) were damaging to function and severely attenuated the capacity of CTF18 to support hematopoiesis and lymphoid development, strongly suggesting that they were responsible for his T lymphopenia; however, the attenuation of CTF18 function appeared to be unrelated to its role as a clamp loader. DNA-damage, expected when replication is impaired, was not evident by expression profiling in murine Chtf18 mutant hematopoietic stem and progenitor cells (HSPC), nor was development of Ctf18-deficient progenitors rescued by p53 loss. Instead, we observed an expression signature suggesting disruption of HSPC positioning and migration. Indeed, the positioning of HSPC in ctf18 morphant zebrafish embryos was perturbed, suggesting that HSPC function was impaired through disrupted positioning in hematopoietic organs. Accordingly, we propose that T lymphopenia in our patient resulted from disturbed cell-cell contacts and migration of HSPC, caused by a non-canonical function of CHTF18 in regulating gene expression.