ORIGINAL RESEARCH article
Front. Immunol.
Sec. Cancer Immunity and Immunotherapy
Volume 16 - 2025 | doi: 10.3389/fimmu.2025.1530583
This article is part of the Research TopicUnveiling the Next Generation of Cancer Immunity & ImmunotherapyView all 12 articles
Effect of bispecific recombinant oncolytic adenovirus carrying apoptin on apoptosis of MCF-7 cells
Provisionally accepted
- 1Jilin Medical University, Jilin, China
- 2Institute of Military Veterinary Medicine, Academy of Military Medical Science, Changchun, Jilin Province, China
- 3Dalian University, Dalian, Liaoning Province, China
- 4Changchun Institute of Applied Chemistry, Chinese Academy of Sciences (CAS), Changchun, Jilin Province, China
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The incidence of breast cancer, a common malignant tumour, is increasing annually worldwide, which is the highest incidence of cancer.Objective: In this study, breast cancer cell line MCF-7 was infected with recombinant oncolytic adenovirus Ad-VT expressing apopsin protein, and its anti-tumor pathway was detected to determine its possible anti-tumor signaling pathway.Method: In this study, the inhibitory effect of recombinant oncolytic adenovirus Ad-VT on breast cancer cells was investigated through cell activity experiment and establishment of tumor bearing model in mice. Subsequently, in order to determine the apoptosis-inducing effect of recombinant oncolytic adenovirus on breast cancer cells, the effects of three recombinant oncolytic adenovirus on the apoptosis-inducing level of breast cancer cells were further analyzed by Annexin V-FITC/PI detection, Hoechst staining, JC-1 staining and transmission electron microscopy. Then the differentially expressed proteins associated with apoptosis and possible signaling pathways were identified by proteomics and WB experiments.In vivo and in vitro experiments showed that recombinant oncolytic adenovirus Ad-VT expressing apoptosis protein could induce apoptosis and inhibit the growth of MCF-7 cells. Proteomic analysis showed that differential genes were enriched in mTOR, MAPK and other pathways after Ad-VT infection of breast cancer cells, and the expression of S6K genes related to mTOR pathway was significantly increased in differential gene analysis, subsequently, the high expression of phosphorylated mTOR and S6K proteins was also determined by WB experiment, suggesting that Ad-VT may regulate the apoptosis of breast cancer cells through mTOR/S6K signaling.Ad-VT can significantly increase the apoptosis level of breast cancer cells, which may be induced by the mTOR/S6K signaling pathway. The results of this study provide a theoretical basis for the development of anti-tumor drugs based on Ad-VT in the future.
Keywords: MCF-7 Cells, Apoptin, Proteomics, Apoptosis, oncolytic adenovirus
Received: 19 Nov 2024; Accepted: 24 Apr 2025.
Copyright: © 2025 Chen, Li, Yin, Hu, Li, Shao, Li and Jin. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Shuang Chen, Jilin Medical University, Jilin, China
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