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ORIGINAL RESEARCH article
Front. Immunol.
Sec. Cancer Immunity and Immunotherapy
Volume 16 - 2025 |
doi: 10.3389/fimmu.2025.1511247
This article is part of the Research Topic Deciphering Cancer Metabolism: A New Frontier in Tumor Immunology with Computational Innovation View all 13 articles
Hsa_circ_0001756 drives gastric cancer glycolysis by increasing the expression and stability of PGK1 mRNA
Provisionally accepted
Long Qian 1
Luman Wang 2
Hao Chen 3* Song Wang 4* Yinfen Hou 4*
Li Xu 4
Yabin Xia 4 Maoqi Xu 5*
xiaoxu Huang 4*- 1 Wuhu Hospital of Traditional Chinese Medicine, Wuhu, Anhui Province, China
- 2 Fudan University, Shanghai, Shanghai Municipality, China
- 3 Hailuo Hospital of Wuhu, wuhu, China
- 4 First Affiliated Hospital of Wannan Medical College, Wuhu, Anhui Province, China
- 5 Anhui Wannan Rehabilitation Hospital, Wuhu, Anhui Province, China
Strategies for preventing high glycolysis in tumour cells are urgently needed.CircRNAs (circRNAs) play important roles in glycolysis. However, the mechanism underlying the effects of hsa_circ_0001756 in gastric cancer (GC) remains unclear. The expression level of hsa_circ_0001756 in GC tissues and cells is significantly upregulated.The expression level of hsa_circ_0001756 is closely related to TNM stage and tumour size in patients with GC. The proliferation and migration of hsa_circ_0001756-expressing cells in vitro were assessed by functional experiments. Hsa_circ_0001756 was found to not only promote the expression and stability of PGK1 by binding with polypyrimidine tract-binding protein 1 (PTBP1) but also promote glycolysis through the miR-185-3P/PGK1 pathway. We found that the regulatory relationships of competing endogenous RNA (ceRNA) and RNAbinding proteins (RBPs) with hsa_circ_0001756may affect glycolysis in GC. This study provides a theoretical basis for designing drugs that target molecules related to energy metabolism in tumours and provides a new strategy for the clinical treatment of GC.
Keywords: circRNA, glycolysis, miR-185-3p, PGK1, PTBP1 CCK8: Cell Counting Kit-8, ceRNAs: competitive endogenous RNAs, circRNA: circular RNA, DFS: disease-free survival, FISH: fluorescence in situ hybridization, GC: gastric cancer, gDNA: genomic DNA, IHC: Immunohistochemistry
Received: 14 Oct 2024; Accepted: 29 Jan 2025.
Copyright: © 2025 Qian, Wang, Chen, Wang, Hou, Xu, Xia, Xu and Huang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Hao Chen, Hailuo Hospital of Wuhu, wuhu, China
Song Wang, First Affiliated Hospital of Wannan Medical College, Wuhu, 241001, Anhui Province, China
Yinfen Hou, First Affiliated Hospital of Wannan Medical College, Wuhu, 241001, Anhui Province, China
Maoqi Xu, Anhui Wannan Rehabilitation Hospital, Wuhu, Anhui Province, China
xiaoxu Huang, First Affiliated Hospital of Wannan Medical College, Wuhu, 241001, Anhui Province, China
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