AI-enhanced profiling of phage-display-identified anti-TIM3 and anti-TIGIT novel antibodies

Astrid Musnier ^1* Yannick Corde ¹ Adrien Verdier ¹ Mélanie Cortes ¹ Christophe Dumet ¹ Jean-René Pallandre ² Adeline Bouard ² Abdelraouf Keskes ¹ Zakaria Omahdi ¹ Vincent Puard ¹ Anne Poupon ¹ Thomas Bourquard ^1*

• ¹ MabSilico, Tours, France
• ² Plateforme ITAC-UMR1098-RIGHT, Établissement Français du Sang (EFS), Besancon, Bourgogne-Franche-Comte, France

Antibody discovery is a lengthy and labor-intensive process, requiring extensive laboratory work to ensure that an antibody demonstrates the appropriate efficacy, production, and safety characteristics necessary for its use as a therapeutic agent in human patients. Traditionally, this process begins with phage display or B-cells isolation campaigns, where affinity serves as the primary selection criterion. However, the initial leads identified through this approach lack sufficient characterization in terms of developability and epitope definition, which are typically performed at late stages. In this study, we present a pipeline that integrates early-stage phage display screenings with AI-based characterization, enabling more informed decision-making throughout the selection process. Using immune checkpoints TIM3 and TIGIT as targets, we identified five initial leads exhibiting similar binding properties. Two of these leads were predicted to have poor developability profiles due to unfavorable surface physicochemical properties. Of the remaining three candidates, structural models of the complexes formed with their respective targets were generated for 2: T4 (against TIGIT) and 6E9 (against TIM3). The predicted epitopes allowed us to anticipate a competition with TIM3 and TIGIT binding partners, and to infer the antagonistic functions expected from these antibodies. This study lays the foundations of a multidimensional AI-driven selection of lead candidates derived from high throughput analysis.