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ORIGINAL RESEARCH article

Front. Immunol.
Sec. Comparative Immunology
Volume 15 - 2024 | doi: 10.3389/fimmu.2024.1521080
This article is part of the Research Topic The Roles of Autophagy and Cell Death in the Host Immune Response in Aquatic Animals View all articles

Pseudomonas plecoglossicida infection induces neutrophil autophagy-driven NETosis in large yellow croaker Larimichthys crocea

Provisionally accepted
Cao Jiafeng Cao Jiafeng Jiong Chen Jiong Chen *
  • Ningbo University, Ningbo, China

The final, formatted version of the article will be published soon.

    Neutrophil extracellular traps (NETs) are crucial for the immune defense of many organisms, serving as a potent mechanism for neutrophils to capture and eliminate extracellular pathogens. While NETosis and its antimicrobial mechanisms have been well studied in mammals, research on NETs formation in teleost fish remains limited.In this study, we used the large yellow croaker (Larimichthys crocea) as the study model to investigate NETosis and its role in pathogen defense. Our results showed that infection with Pseudomonas plecoglossicida could induce NETosis. To further explore the underlying mechanism, we performed transcriptome analysis and western blotting, which revealed that P. plecoglossicida triggers NETosis through activation of the autophagy pathway. Inhibition of autophagy significantly reduced NET production, highlighting its critical role in this process. Furthermore, our studies demonstrated that NETs exert a bacteriostatic effect, significantly suppressing the growth of P. plecoglossicida. Taken together, our findings reveal that autophagy regulates NETosis in large yellow croaker and underscore the essential role of NETs in bacterial defense, providing new insights into immune responses in teleost fish.

    Keywords: Pseudomonas plecoglossicida, Larimichthys crocea, neutrophil extracellular traps, Autophagy, Antibacterial

    Received: 01 Nov 2024; Accepted: 22 Nov 2024.

    Copyright: © 2024 Jiafeng and Chen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Jiong Chen, Ningbo University, Ningbo, China

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