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ORIGINAL RESEARCH article

Front. Immunol.
Sec. Microbial Immunology
Volume 15 - 2024 | doi: 10.3389/fimmu.2024.1520296

Synergistic defecation effects of Bifidobacterium animalis subsp. lactis BL-99 and fructooligosaccharide by modulating gut microbiota

Provisionally accepted
Qi Zhang Qi Zhang 1Wen Zhao Wen Zhao 1Jie Luo Jie Luo 2Shaoqi Shi Shaoqi Shi 1Xiaokang Niu Xiaokang Niu 1Jian He Jian He 3Yicheng Wang Yicheng Wang 1Zhaozhong Zeng Zhaozhong Zeng 3Qiuyue Jiang Qiuyue Jiang 4Bing Fang Bing Fang 1Juan Chen Juan Chen 1Yixuan Li Yixuan Li 1Fuqing Wang Fuqing Wang 5Jingjing He Jingjing He 1Jie Guo Jie Guo 1Ming Zhang Ming Zhang 6Liwei Zhang Liwei Zhang 1Shaoyang Ge Shaoyang Ge 7Wei-Lian Hung Wei-Lian Hung 3Ran Wang Ran Wang 1*
  • 1 China Agricultural University, Beijing, China
  • 2 Hunan Agricultural University, Changsha, Hunan, China
  • 3 Inner Mongolia National Center of Technology Innovation for Dairy Co. Ltd., Hohhot, China
  • 4 Yili Innovation Center, Inner Mongolia Yili Industrial Group Co., Ltd., Hohhot, Inner Mongolia Autonomous Region, China
  • 5 Tibet Tianhong Science and Technology Co., Ltd., Lhasa, China
  • 6 School of Food and Health, Beijing Technology and Business University, Beijing, China
  • 7 Hebei Engineering Research Center of Animal Product, Sanhe, China

The final, formatted version of the article will be published soon.

    Synbiotics have revealed the possibility of improving constipation through gut microbiota. The synergistic efficacy of Bifidobacterium animalis subsp. lactis BL-99 (BL-99) and fructooligosaccharide (FOS) on constipation have not been investigated. In this study, loperamide-induced constipated mice model was established to explore the effect of BL-99, FOS, and BL-99+FOS on changes of defecationrelated parameters, gut microbiota and metabolites. The results showed that BL-99, FOS, and BL-99+FOS each alleviated constipation, with the synbiotic showing significant efficacy in the first black stool defecation time, fecal number, fecal weight, and the gastrointestinal transit rate (P < 0.05).Additionally, significant increased in serum 5-HT and IL-10 were observed in the BL-99+FOS group, alongside an increased relative abundance of Lachnospiraceae_NK4A136_group, Blautia, and Clostridium sensu stricto 1, while significantly reducing the relative abundance of Alistipes and Bacteroides. These changes facilitated alterations in short-chain fatty acids (SCFAs) metabolism, and were closely associated with the expression of genes related to the 5-HT pathway and the modulation of serum inflammatory factors. This study provides a theoretical basis for BL-99 and FOS synbiotics to improve constipation by regulating the gut microbiota and metabolites.

    Keywords: Bifidobacterium animalis subsp. lactis BL-99, Fructo-oligosaccharide, Gut Microbiota, Constipation, Serum inflammatory factors

    Received: 31 Oct 2024; Accepted: 18 Dec 2024.

    Copyright: © 2024 Zhang, Zhao, Luo, Shi, Niu, He, Wang, Zeng, Jiang, Fang, Chen, Li, Wang, He, Guo, Zhang, Zhang, Ge, Hung and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Ran Wang, China Agricultural University, Beijing, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.