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ORIGINAL RESEARCH article

Front. Immunol.
Sec. Cancer Immunity and Immunotherapy
Volume 15 - 2024 | doi: 10.3389/fimmu.2024.1511824
This article is part of the Research Topic Precision Immunotherapy and Novel Target Discovery in Hematological Malignancy View all 4 articles

Identification of Hub Genes and immune-related Pathways in Acute Myeloid Leukemia: Insights from Bioinformatics and Experimental Validation

Provisionally accepted
Mingliang Shan Mingliang Shan 1,2,3Li Xu Li Xu 4Wenzhe Yang Wenzhe Yang 5Shiguo Liu Shiguo Liu 2Zhaoqing Cui Zhaoqing Cui 1*
  • 1 Liaocheng People's Hospital, Liaocheng, China
  • 2 The Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China
  • 3 Gaomi Maternal and Child Health Hospital, Weifang City, China
  • 4 Shandong Second Medical University, Weifang, China
  • 5 Shandong University of Traditional Chinese Medicine, Jinan, Shandong Province, China

The final, formatted version of the article will be published soon.

    Background:This study aims to identify the hub genes and immune-related pathways in acute myeloid leukemia (AML) to provide new theories for immunotherapy.We use bioinformatics methods to find and verify the hub gene. At the same time, we use the results of GSEA enrichment analysis to find immune-related mediators. Through Mendelian randomization(MR) analysis, on the one hand, we look for related immune cells, and on the other hand, we use it to determine the causal relationship among immune cells, immune mediators, and AML. Finally, in vitro experiments are conducted to further verify and improve the reliability and physiological functions of the hub gene and its immune-related pathways.Results: Complement Factor D(CFD) gene is identified as the highly expressed hub gene and is positively correlated with IL-2. IL-2 is also positively correlated with CD27 on CD24+CD27+B cells, JAK/STAT, and PI3K/Akt. The latter three are positively correlated with the occurrence and development of AML.We conclude that CFD gene uses IL-2 as a mediator to promote the disease progression of AML by promoting the CD27 on CD24+CD27+B cells, JAK-STAT, and PI3K-Akt pathways.

    Keywords: AML1, hub gene2, Bioinformatics3, machine learning4, Mendelian randomization5, plasmid6, RT-qPCR7

    Received: 15 Oct 2024; Accepted: 24 Dec 2024.

    Copyright: © 2024 Shan, Xu, Yang, Liu and Cui. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Zhaoqing Cui, Liaocheng People's Hospital, Liaocheng, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.