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ORIGINAL RESEARCH article

Front. Immunol.
Sec. Vaccines and Molecular Therapeutics
Volume 15 - 2024 | doi: 10.3389/fimmu.2024.1511526
This article is part of the Research Topic Immunomodulatory Bioactive Hydrogels for the Repair and Rehabilitation of Anisotropic Tissues View all articles

Promotion of Angiogenesis and Suppression of Inflammatory Response in Skin Wound Healing Using Exosome-Loaded Collagen Sponge

Provisionally accepted
Siqi Zhang Siqi Zhang 1Xugang Lu Xugang Lu 1Jun Chen Jun Chen 1Shibing Xiong Shibing Xiong 1Yifan Cui Yifan Cui 1Simeng Wang Simeng Wang 1Chongxia Yue Chongxia Yue 1Han Qianqian Han Qianqian 2Bangcheng Yang Bangcheng Yang 1*
  • 1 Sichuan University, Chengdu, China
  • 2 National Institutes for Food and Drug Control (China), Beijing, Beijing Municipality, China

The final, formatted version of the article will be published soon.

    Effectively promoting skin wound healing remains a significant challenge in the medical field. Although stem cell-derived exosomes show potential in tissue regeneration, their local delivery and sustained release face challenges. To address these issues, we developed a collagen sponge based on type I and recombinant humanized type III collagen. Our study confirmed that exosomes were successfully loaded onto the sponge (sponge-Exo) and the sponge-Exo gradually released exosomes into the local milieu. The sponge-Exo played a crucial role in promoting the transition of macrophages from an inflammatory M1 phenotype to a regenerative M2 phenotype. Moreover, it enhanced the migration and proliferation of HDFs and promoted angiogenesis in HUVECs. Additionally, our findings revealed that the sponge-Exo accelerated wound healing by suppressing inflammatory response and stimulating angiogenesis in a rat full-thickness skin wounds model. Next generation sequencing (NGS) was used to explore the underlying mechanism of wound healing, and the results showed that the miRNAs (hsa-miR-21-5p and hsa-miR-29a-5p) associated with wound healing in exosomes were significantly up-regulated. These results highlight the remarkable effects of sponge-Exo on macrophage transformation, cell migration, proliferation and angiogenesis, which provide a potential prospect for the application in the field of skin wound healing.

    Keywords: Exosomes, collagen sponge, Angiogenesis, Inflammatory response modulation, Wound Healing

    Received: 15 Oct 2024; Accepted: 13 Nov 2024.

    Copyright: © 2024 Zhang, Lu, Chen, Xiong, Cui, Wang, Yue, Qianqian and Yang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Bangcheng Yang, Sichuan University, Chengdu, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.