Skip to main content

ORIGINAL RESEARCH article

Front. Immunol.
Sec. Viral Immunology
Volume 15 - 2024 | doi: 10.3389/fimmu.2024.1505840

Massive endocytosis mechanisms are involved in uptake of HIV-1 viral particles by monocyte-derived dendritic cells

Provisionally accepted
Fernando Laguía Fernando Laguía 1Jakub Chojnacki Jakub Chojnacki 1Itziar Erkizia Itziar Erkizia 1María Isabel Geli María Isabel Geli 2Carlos Enrich Carlos Enrich 3Javier Martinez-Picado Javier Martinez-Picado 1*Patricia Resa-Infante Patricia Resa-Infante 1*
  • 1 IrsiCaixa, Barcelona, Spain
  • 2 Institute of Molecular Biology of Barcelona, Spanish National Research Council (CSIC), Barcelona, Catalonia, Spain
  • 3 University of Barcelona, Barcelona, Catalonia, Spain

The final, formatted version of the article will be published soon.

    HIV-1 exploits dendritic cells (DCs) to spread throughout the body via specific recognition of gangliosides present on the viral envelope by the CD169/Siglec-1 membrane receptor. This interaction triggers the internalization of HIV-1 within a structure known as the sac-like compartment.While the mechanism underlying sac-like compartment formation remains elusive, prior research indicates that the process is clathrin-independent and cell membrane cholesterol-dependent and involves transient disruption of cortical actin. Here, we investigate the potential involvement of massive endocytosis (MEND) in this process. Our data demonstrate extensive plasma membrane invagination based on sac-like compartment dimensions (2.9 μm in diameter and 20 μm 3 in volume). We showed that the cholesterol concentration doubles within the regions of viral uptake, suggesting lipid-phase separation, and that development of the sac-like compartment is accompanied by transient depolarization of cortical actin. Moreover, we observed that protein palmitoylation and PI3K inhibition reduce the sac-like compartment formation rate from 70% to 20% and 40%, respectively. These results indicate the involvement of MEND mechanisms during sac-like compartment formation.

    Keywords: Dendritic Cells, CD169/Siglec1, HIV, sac-like compartment, MEND

    Received: 03 Oct 2024; Accepted: 11 Dec 2024.

    Copyright: © 2024 Laguía, Chojnacki, Erkizia, Geli, Enrich, Martinez-Picado and Resa-Infante. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Javier Martinez-Picado, IrsiCaixa, Barcelona, Spain
    Patricia Resa-Infante, IrsiCaixa, Barcelona, Spain

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.