Corrigendum: Cytosolic protein translation regulates cell asymmetry and function in early TCR activation of human CD8+ T lymphocytes

A Corrigendum on
Cytosolic protein translation regulates cell asymmetry and function in early TCR activation of human CD8⁺ T lymphocytes

By Gómez-Morón Á, Tsukalov I, Scagnetti C, Pertusa C, Lozano-Prieto M, Martínez-Fleta P, Requena S, Martín P, Alfranca A, Martin-Gayo E and Martin-Cofreces NB (2024) Front. Immunol. 15:1411957. doi: 10.3389/fimmu.2024.1411957

In the published article, there was an error. The name “70S” was used to name mitochondrial ribosomes instead of “55S”.

A correction has been made to Abstract, Introduction. This sentence previously stated:

“Mitochondria play a role in regulating CTL function by controlling processes such as calcium flux, providing the necessary energy through oxidative phosphorylation, and its own protein translation on 70S ribosomes.”

The corrected sentence appears below:

“Mitochondria play a role in regulating CTL function by controlling processes such as calcium flux, providing the necessary energy through oxidative phosphorylation, and its own protein translation on 55S ribosomes.”

A correction has also been made to Abstract, Methods. This sentence previously stated:

“Here, we investigated the importance of cytosolic protein synthesis in human CTLs after early TCR activation and CD28 co-stimulation for the dynamic reorganisation of the cytoskeleton, mitochondria, and lytic granules through short-term chemical inhibition of 80S ribosomes by cycloheximide and 80S and 70S by puromycin.”

The corrected sentence appears below:

“Here, we investigated the importance of cytosolic protein synthesis in human CTLs after early TCR activation and CD28 co-stimulation for the dynamic reorganisation of the cytoskeleton, mitochondria, and lytic granules through short-term chemical inhibition of 80S ribosomes by cycloheximide and 80S and 55S by puromycin.”

A correction has been made to Introduction, Paragraph 3. This sentence previously stated:

“CTLs require correct translation at mitochondrial 70S ribosomes to help CD8+ cytotoxic T cell-mediated prolonged killing (39).”

The corrected sentence appears below:

“CTLs require correct translation at mitochondrial 55S ribosomes to help CD8+ cytotoxic T cell-mediated prolonged killing (39).”

A correction has been made to Results, Eukaryotic protein translation inhibition affects tubulin cytoskeleton dynamics and TCR signalling of human cytotoxic T lymphocytes, Paragraph 1. This sentence previously stated:

“Indeed, PURO has also been used to inhibit mitochondrial 70S ribosomes in vitro (47).”

The corrected sentence appears below:

“Indeed, PURO has also been used to inhibit mitochondrial 55S ribosomes in vitro (47).”

A correction has also been made to Results, Eukaryotic protein translation inhibition affects tubulin cytoskeleton dynamics and TCR signalling of human cytotoxic T lymphocytes, Paragraph 3. This sentence previously stated:

“Indeed, inhibition of 70S mitochondrial ribosomes could also increase the effect in CTLs after puromycin treatment.”

The corrected sentence appears below:

“Indeed, inhibition of 55S mitochondrial ribosomes could also increase the effect in CTLs after puromycin treatment.”

A correction has been made to Discussion, Paragraph 1. This sentence previously stated:

“A site is used by puromycin to enter the ribosome and bind, causing rapid chain termination by covalently attaching to the C-terminus of the nascent chain, producing a Cterminal puromycylated polypeptide (46); puromycin is also known to act in the 80S and 70S ribosomes (47).”

The corrected sentence appears below:

“A site is used by puromycin to enter the ribosome and bind, causing rapid chain termination by covalently attaching to the C-terminus of the nascent chain, producing a Cterminal puromycylated polypeptide (46); puromycin is also known to act in the 80S and 55S ribosomes (47).”

A correction has also been made to Discussion, Paragraph 6. This sentence previously stated:

“Here, we demonstrate the relevant role of cytosolic protein translation in facilitating human CTL functionality that is probably potentiated by mitochondrial ribosomes, as deduced by the potential effect of puromycin on 70S ribosomes.”

The corrected sentence appears below:

“Here, we demonstrate the relevant role of cytosolic protein translation in facilitating human CTL functionality that is probably potentiated by mitochondrial ribosomes, as deduced by the potential effect of puromycin on 55S ribosomes.”

The authors apologize for these errors and state that they do not change the scientific conclusions of the article in any way. The original article has been updated.

Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.