This article is a correction to:
Cytosolic protein translation regulates cell asymmetry and function in early TCR activation of human CD8+ T lymphocytes
Álvaro Gómez-Morón1,2
Ilya Tsukalov1,3Camila Scagnetti1,4
Clara Pertusa1Marta Lozano-Prieto1,5
Pedro Martínez-Fleta1Silvia Requena1
Pilar Martín5,6
Aranzazu Alfranca1,3,5
Enrique Martin-Gayo1,3,7
Noa B Martin-Cofreces1,4,5,8*- 1Immunology Service, Instituto de Investigación Sanitaria del Hospital Universitario La Princesa, IIS- Princesa, Universidad Autónoma de Madrid (UAM), Madrid, Spain
- 2Department of Immunology, Ophthalmology and ENT, School of Medicine, Universidad Complutense de Madrid and 12 de Octubre Health Research Institute (imas12), Madrid, Spain
- 3Medicine Faculty, Universidad Autónoma de Madrid (UAM), Madrid, Spain
- 4Videomicroscopy Unit, Instituto de Investigación Sanitaria del Hospital Universitario La Princesa, IIS-Princesa, Universidad Autónoma de Madrid (UAM), Madrid, Spain
- 5Centro de Investigación Biomédica en Red de Enfermedades Cardiovasculares (CIBERCV), Instituto de Salud Carlos III, Madrid, Spain
- 6Area of Vascular Pathophysiology, Laboratory of Regulatory Molecules of Inflammatory Processes, Fundación Centro Nacional de Investigaciones Cardiovasculares-Carlos III, Madrid, Spain
- 7Centro de Investigación Biomédica en Red Enfermedades Infecciosas (CIBERINFECC), Instituto de Salud Carlos III, Madrid, Spain
- 8Area of Vascular Pathophysiology, Laboratory of Intercellular Communication, Fundación Centro Nacional de Investigaciones Cardiovasculares-Carlos III, Madrid, Spain
A Corrigendum on
Cytosolic protein translation regulates cell asymmetry and function in early TCR activation of human CD8+ T lymphocytes
By Gómez-Morón Á, Tsukalov I, Scagnetti C, Pertusa C, Lozano-Prieto M, Martínez-Fleta P, Requena S, Martín P, Alfranca A, Martin-Gayo E and Martin-Cofreces NB (2024) Front. Immunol. 15:1411957. doi: 10.3389/fimmu.2024.1411957
In the published article, there was an error. The name “70S” was used to name mitochondrial ribosomes instead of “55S”.
A correction has been made to Abstract, Introduction. This sentence previously stated:
“Mitochondria play a role in regulating CTL function by controlling processes such as calcium flux, providing the necessary energy through oxidative phosphorylation, and its own protein translation on 70S ribosomes.”
The corrected sentence appears below:
“Mitochondria play a role in regulating CTL function by controlling processes such as calcium flux, providing the necessary energy through oxidative phosphorylation, and its own protein translation on 55S ribosomes.”
A correction has also been made to Abstract, Methods. This sentence previously stated:
“Here, we investigated the importance of cytosolic protein synthesis in human CTLs after early TCR activation and CD28 co-stimulation for the dynamic reorganisation of the cytoskeleton, mitochondria, and lytic granules through short-term chemical inhibition of 80S ribosomes by cycloheximide and 80S and 70S by puromycin.”
The corrected sentence appears below:
“Here, we investigated the importance of cytosolic protein synthesis in human CTLs after early TCR activation and CD28 co-stimulation for the dynamic reorganisation of the cytoskeleton, mitochondria, and lytic granules through short-term chemical inhibition of 80S ribosomes by cycloheximide and 80S and 55S by puromycin.”
A correction has been made to Introduction, Paragraph 3. This sentence previously stated:
“CTLs require correct translation at mitochondrial 70S ribosomes to help CD8+ cytotoxic T cell-mediated prolonged killing (39).”
The corrected sentence appears below:
“CTLs require correct translation at mitochondrial 55S ribosomes to help CD8+ cytotoxic T cell-mediated prolonged killing (39).”
A correction has been made to Results, Eukaryotic protein translation inhibition affects tubulin cytoskeleton dynamics and TCR signalling of human cytotoxic T lymphocytes, Paragraph 1. This sentence previously stated:
“Indeed, PURO has also been used to inhibit mitochondrial 70S ribosomes in vitro (47).”
The corrected sentence appears below:
“Indeed, PURO has also been used to inhibit mitochondrial 55S ribosomes in vitro (47).”
A correction has also been made to Results, Eukaryotic protein translation inhibition affects tubulin cytoskeleton dynamics and TCR signalling of human cytotoxic T lymphocytes, Paragraph 3. This sentence previously stated:
“Indeed, inhibition of 70S mitochondrial ribosomes could also increase the effect in CTLs after puromycin treatment.”
The corrected sentence appears below:
“Indeed, inhibition of 55S mitochondrial ribosomes could also increase the effect in CTLs after puromycin treatment.”
A correction has been made to Discussion, Paragraph 1. This sentence previously stated:
“A site is used by puromycin to enter the ribosome and bind, causing rapid chain termination by covalently attaching to the C-terminus of the nascent chain, producing a Cterminal puromycylated polypeptide (46); puromycin is also known to act in the 80S and 70S ribosomes (47).”
The corrected sentence appears below:
“A site is used by puromycin to enter the ribosome and bind, causing rapid chain termination by covalently attaching to the C-terminus of the nascent chain, producing a Cterminal puromycylated polypeptide (46); puromycin is also known to act in the 80S and 55S ribosomes (47).”
A correction has also been made to Discussion, Paragraph 6. This sentence previously stated:
“Here, we demonstrate the relevant role of cytosolic protein translation in facilitating human CTL functionality that is probably potentiated by mitochondrial ribosomes, as deduced by the potential effect of puromycin on 70S ribosomes.”
The corrected sentence appears below:
“Here, we demonstrate the relevant role of cytosolic protein translation in facilitating human CTL functionality that is probably potentiated by mitochondrial ribosomes, as deduced by the potential effect of puromycin on 55S ribosomes.”
The authors apologize for these errors and state that they do not change the scientific conclusions of the article in any way. The original article has been updated.
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Keywords: protein translation, cell asymmetry, cytotoxic CD8+ T lymphocytes, cytoskeleton, metabolism, mitochondria, T cell activation, immunological synapse
Citation: Gómez-Morón Á, Tsukalov I, Scagnetti C, Pertusa C, Lozano-Prieto M, Martínez-Fleta P, Requena S, Martín P, Alfranca A, Martin-Gayo E and Martin-Cofreces NB (2024) Corrigendum: Cytosolic protein translation regulates cell asymmetry and function in early TCR activation of human CD8+ T lymphocytes. Front. Immunol. 15:1500666. doi: 10.3389/fimmu.2024.1500666
Received: 23 September 2024; Accepted: 30 September 2024;
Published: 14 October 2024.
Edited and Reviewed by:
Michael Loran Dustin, University of Oxford, United KingdomCopyright © 2024 Gómez-Morón, Tsukalov, Scagnetti, Pertusa, Lozano-Prieto, Martínez-Fleta, Requena, Martín, Alfranca, Martin-Gayo and Martin-Cofreces. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Noa B Martin-Cofreces, noa.martin@salud.madrid.org