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METHODS article

Front. Immunol.
Sec. Molecular Innate Immunity
Volume 15 - 2024 | doi: 10.3389/fimmu.2024.1493848
This article is part of the Research Topic Methods in Molecular Innate Immunity: 2022 View all 8 articles

Analysis of lipid uptake, storage, and fatty acid oxidation by group 2 innate lymphoid cells

Provisionally accepted
Audrey Roy-Dorval Audrey Roy-Dorval 1,2,3Rebecca C. Deagle Rebecca C. Deagle 1,2,3Frederik Roth Frederik Roth 1,2,3Mathilde Raybaud Mathilde Raybaud 1,2,3Nailya Ismailova Nailya Ismailova 1,2,3Sai Sakktee Krisna Sai Sakktee Krisna 3,4,5,6Damon G. Aboud Damon G. Aboud 7Camille Stegen Camille Stegen 2,3Julien Leconte Julien Leconte 2,3Gabriel Berberi Gabriel Berberi 1,2,3Ademola Esomojumi Ademola Esomojumi 1,2,3Jörg H. Fritz Jörg H. Fritz 1,3,4,8*
  • 1 Department of Microbiology and Immunology, School of Biomedical Sciences, Faculty of Medicine and Health Sciences, McGill University, Montreal, Quebec, Canada
  • 2 Department of Microbiology and Immunology, McGill University Research Center on Complex Traits (MRCCT), McGill University, Québec, Canada, Montreal, Ontario, Canada
  • 3 Dahdaleh Institute of Genomic Medicine (DIgM), McGill University, Montreal, Canada
  • 4 Department of Physiology, School of Biomedical Sciences, Faculty of Medicine and Health Sciences, McGill University, Montreal, Quebec, Canada
  • 5 McGill University Research Center on Complex Traits (MRCCT), McGill University, Montreal, Canada
  • 6 Segal Cancer Centre, Lady Davis Institute for Medical Research, Jewish General Hospital, McGill University, Montreal, Canada
  • 7 Department of Chemical Engineering, McGill University, Montreal, Canada
  • 8 Complex Traits Group, Microbiology and Immunology,, McGill University, Montreal, Quebec, Canada

The final, formatted version of the article will be published soon.

    We are submitting the attached manuscript entitled, "Analysis of lipid uptake, storage, and fatty acid oxidation by group 2 innate lymphoid cells" by Roy-Dorval et al. for consideration of publication in Frontiers in Immunology.Group 2 Innate Lymphoid Cells (ILC2) are critical drivers of both innate and adaptive type 2 immune responses, known to orchestrate processes involved in tissue restoration and wound healing. In addition, ILC2 have been implicated in chronic inflammatory barrier disorders in type 2 immunopathologies such as allergic rhinitis and asthma. ILC2 in the context of allergen-driven airway inflammation have recently been shown to influence local and systemic metabolism, as well as being rich in lipid-storing organelles called lipid droplets. However, mechanisms of ILC2 lipid anabolism and catabolism remain largely unknown and the impact of these metabolic processes in regulating ILC2 phenotypes and effector functions has not been extensively characterized. ILC2 phenotypes and effector functions are shaped by their metabolic status, and determining the metabolic requirements of ILC2 is critical in understanding their role in type 2 immune responses and their associated pathophysiology. We detail here a novel experimental method of implementing flow cytometry for large scale analysis of fatty acid uptake, storage of neutral lipids, and fatty acid oxidation in primary murine ILC2 with complementary morphological analysis of lipid storage using confocal microscopy. By combining flow cytometry and confocal microscopy, we can identify the metabolic lipid requirements for ILC2 functions as well as Analysis of lipid uptake, storage, and fatty acid oxidation by group 2 innate lymphoid cells

    Keywords: Group 2 innate lymphoid cells (ILC2), Type 2 immunity, Immunometabolism, fatty acid uptake, lipid droplets, Fatty acid oxidation (FAO)

    Received: 09 Sep 2024; Accepted: 24 Sep 2024.

    Copyright: © 2024 Roy-Dorval, Deagle, Roth, Raybaud, Ismailova, Krisna, Aboud, Stegen, Leconte, Berberi, Esomojumi and Fritz. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Jörg H. Fritz, Complex Traits Group, Microbiology and Immunology,, McGill University, Montreal, H3G0B1, Quebec, Canada

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