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ORIGINAL RESEARCH article

Front. Immunol.
Sec. Vaccines and Molecular Therapeutics
Volume 15 - 2024 | doi: 10.3389/fimmu.2024.1490260
This article is part of the Research Topic Innovative Adjuvant Strategies: Enhancing Vaccine Efficacy Through Transdisciplinary Approaches View all articles

Pomacea canaliculata hemocyanin as a novel natural immunostimulant in mammals

Provisionally accepted
Ignacio Rafael Chiumiento Ignacio Rafael Chiumiento 1,2María Alejandra Tricerri María Alejandra Tricerri 1,3María Fernanda Cortez María Fernanda Cortez 1Santiago Ituarte Santiago Ituarte 1,3Julia Tau Julia Tau 1Karina Valeria Mariño Karina Valeria Mariño 4Paola Lorena Smaldini Paola Lorena Smaldini 5,6Horacio Heras Horacio Heras 1,2Marcos Sebastián Dreon Marcos Sebastián Dreon 1,3*
  • 1 Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP), La Plata, Argentina
  • 2 Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, La Plata, Argentina
  • 3 Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina
  • 4 Instituto de Biología y Medicina Experimental (IBYME), Buenos Aires, Argentina., Buenos Aires, Argentina
  • 5 CONICET Instituto de Estudios Inmunológicos y Fisiopatalógicos (IIFP), La Plata, Buenos Aires, Argentina
  • 6 Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina

The final, formatted version of the article will be published soon.

    Gastropod hemocyanins are potent immunostimulants in mammals, a trait associated with their large molecular size and unusual glycosylation patterns. While the hemocyanin from the marine snail keyhole limpet (KLH), has been widely studied and successfully employed as a carrier/adjuvant in several immunological applications, as well as a nonspecific immunostimulant for bladder cancer treatment, few other gastropod hemocyanins have been biochemically and immunologically characterized. In this work, we investigated the immunogenic properties of the hemocyanin an from Pomacea canaliculata (PcH), an invasive south American freshwater snail. This species, known for its highpreproductive rate and easy rearing, represents a promising source of potential biomedical compounds, including hemocyanin. Employing flow cytometry, fluorescence microscopy, immunoassays, and quantitative PCR, we analysed the effects of PcH on THP-1 monocytes and their derived macrophages, as well as its ability to induce humoral response on C57BL/6 mice. Additionally, we evaluated the structural stability of PcH across a wide range of temperature and pH values. Our findings demonstrate that PcH is a structurally stable protein that not only triggers a pro-inflammatory effect on THP-1 derived-macrophages by increasing IL1-β and TNF-α levels, but also promotes phenotipyc changes associated with the monocyte-to-macrophage differentiation.Moreover, the humoral response induced by PcH in mice was indistinguishable from that of KLH, highlighting the promising immunostimulatory properties of this freshwater snail hemocyanin. Con formato: Sin Resaltar Con formato: Sin Resaltar Con formato: Inglés (Estados Unidos) Con formato: Inglés (Estados Unidos) Con formato: Inglés (Estados Unidos

    Keywords: Gastropods, mollusc, Respiratory pigment, innate immunity, macrophage

    Received: 02 Sep 2024; Accepted: 16 Dec 2024.

    Copyright: © 2024 Chiumiento, Tricerri, Cortez, Ituarte, Tau, Mariño, Smaldini, Heras and Dreon. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Marcos Sebastián Dreon, Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP), La Plata, Argentina

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