AUTHOR=Zhu Yiqing , Liang Xiao , Zhi Mengfan , Li Lixiang , Zhang Guoming , Chen Changxu , Wang Limei , Wang Peng , Zhong Ning , Feng Qiang , Li Zhen 

TITLE=Succession of the multi-site microbiome along pancreatic ductal adenocarcinoma tumorigenesis

JOURNAL=Frontiers in Immunology

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2024.1487242

DOI=10.3389/fimmu.2024.1487242

ISSN=1664-3224

ABSTRACT=<sec><title>Background</title><p>To investigate microbial characteristics across multibody sites from chronic pancreatitis (CP), through pancreatic benign tumors, to pancreatic ductal adenocarcinoma (PDAC) at different stages.</p></sec><sec><title>Methods</title><p>16S ribosomal RNA (rRNA) amplicon sequencing was conducted on saliva, duodenal fluid, and pancreatic tissue obtained via endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) of patients with CP, pancreatic benign tumors, PDAC in stage I/II, III, and IV. The neutral community model (NCM) assessed the microbial assembly contribution and MaAslin2 identified the differential microbes.</p></sec><sec><title>Results</title><p>From CP to stage IV PDAC patients, there was a marked surge in influence of salivary and duodenal microbiota on constitution of pancreatic microbial communities. Our observations revealed a successive alteration in microbial species across various bodily sites during PDAC tumorigenesis. Notably, <italic>Porphyromonas gingivalis</italic>, <italic>Treponema denticola</italic>, <italic>Peptoanaerobacter stomatis</italic>, <italic>Propionibacterium acidifaciens</italic>, <italic>Porphyromonas endodontalis</italic>, <italic>Filifactor alocis</italic>, etc., sequentially increased along PDAC progression in pancreatic tissue, whereas bacteria commonly used as probiotics <italic>Bifidobacterium breve</italic>, <italic>Lactiplantibacillus plantarum</italic>, etc., declined. Furthermore, the sequentially escalating trends of <italic>Peptoanaerobacter stomatis</italic> and <italic>Propionibacterium acidifaciens</italic> during PDAC tumorigenesis were mirrored in duodenal fluid and saliva. <italic>Porphyromonas gingivalis</italic>, <italic>Porphyromonas endodontalis</italic>, and <italic>Filifactor alocis</italic>, which intensified from CP to stage IV PDAC in pancreatic tissue, were also found to be enriched in saliva of patients with short-term survival (STS) compared with those with long-term survival (LTS).</p></sec><sec><title>Conclusions</title><p>Salivary and duodenal microorganisms were prominent factors in shaping pancreatic microbial landscape in PDAC context. Further exploration of these microbial entities is imperative to unravel their specific roles in PDAC pathogenesis, potentially yielding insights for future therapeutic strategies.</p></sec>