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ORIGINAL RESEARCH article

Front. Immunol.
Sec. Comparative Immunology
Volume 15 - 2024 | doi: 10.3389/fimmu.2024.1480897
This article is part of the Research Topic New Progress of Nutritional Immunity on Aquatic Animals by Functional Feed Additives under the Condition of Low Fish Meal View all 9 articles

Supplementation of Yupingfeng polysaccharides in low fishmeal diets enhances intestinal health through influencing the intestinal barrier, immunity, and microflora in Macrobrachium rosenbergii

Provisionally accepted
Mingyang Liu Mingyang Liu 1*Cunxin Sun Cunxin Sun 2Qunlan Zhou Qunlan Zhou 2Pao Xu Pao Xu 2Xiaochuan Zheng Xiaochuan Zheng 2Bo Liu Bo Liu 2
  • 1 Nanjing Agricultural University, Nanjing, China
  • 2 Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences (CAFS), Wuxi, Jiangsu Province, China

The final, formatted version of the article will be published soon.

    This study investigated the effects of a low-fishmeal diet (LF, substituting soybean meal for 40% fish meal) and the supplementation of 500 mg/kg and 1000 mg/kg Yu Ping Feng (YPF) polysaccharides on the growth performance, antioxidant enzyme activities, intestinal ultrastructure and non-specific immunity, and microbiota of Macrobrachium rosenbergii. Results indicated that the LF diet significantly reduced the growth performance parameters compared to the control group, while YPF supplementation notably improved these parameters, especially at a 1000 mg/kg dosage. Antioxidant enzyme activities (SOD, GSH-PX) were diminished in the LF group, accompanied by elevated MDA levels, whereas YPF supplementation restored these activities and reduced MDA levels. Ultrastructural analysis revealed that the LF diet caused intestinal villi detachment and peritrophic matrix (PM) shedding, which were alleviated by YPF. Gene expression related to PM formation (GS, CHS, EcPT) was downregulated in the LF group but significantly upregulated in the 1000P group.Non-specific immune gene expressions (IMD, Relish, IκBα) and enzyme activities (NO, iNOS) were suppressed in the LF group but enhanced by YPF supplementation.Microbial community analysis showed reduced diversity and altered composition in the LF group, with increased Proteobacteria and decreased Firmicutes, which were partially restored by YPF. Correlation analysis revealed that Lactobacillus and Chitinibacter play pivotal roles in regulating intestinal health. Lactobacillus exhibited a positive relationship with the intestinal PM and immune-related indicators, whereas Chitinibacter was negatively associated with these factors.These results highlight the adverse impacts of a LF diet on the intestinal health of M. rosenbergii and demonstrate the beneficial effects of YPF polysaccharides in alleviating these negative consequences through various mechanisms.

    Keywords: Yupingfeng polysaccharides, alternative fish meal diet, peritrophic matrix, intestinal immunity, Intestinal microbes, Macrobrachium rosenbergii

    Received: 14 Aug 2024; Accepted: 04 Nov 2024.

    Copyright: © 2024 Liu, Sun, Zhou, Xu, Zheng and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Mingyang Liu, Nanjing Agricultural University, Nanjing, China

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