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ORIGINAL RESEARCH article

Front. Immunol.
Sec. Cancer Immunity and Immunotherapy
Volume 15 - 2024 | doi: 10.3389/fimmu.2024.1467098
This article is part of the Research Topic Genetics and Epigenetics of Melanoma and Non-Melanoma Skin Cancer View all articles

Research on the Influence of Radiotherapy-Related Genes on Immune Infiltration, Immunotherapy Response and Prognosis in Melanoma based on multi-omics

Provisionally accepted
Yujing Shi Yujing Shi 1Wantong Zhao Wantong Zhao 2Yuanjian Ding Yuanjian Ding 3Xiaolin Ge Xiaolin Ge 4Mengyang Ju Mengyang Ju 3*
  • 1 Jurong People's Hospital, Jurong, China
  • 2 Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning Province, China
  • 3 Osaka University, Suita, Ōsaka, Japan
  • 4 First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu Province, China

The final, formatted version of the article will be published soon.

    Background: Skin cutaneous melanoma (SKCM) is a significant oncological challenge due to its aggressive nature and poor treatment outcomes. This study explores the comprehensive effects of radiotherapy (RT) in SKCM, focusing on cell signaling pathways, immune infiltration, immune gene correlations, immunotherapy response, and prognosis. Methods: Using the Cancer Genome Atlas (TCGA) database, differentially expressed genes (DEGs) in SKCM patients undergoing RT were identified. A risk score model based on these DEGs was developed to assess the effects of RT-related genes on drug sensitivity, immune cell infiltration, immunotherapy response, and prognosis through multi-omics analysis. Human melanoma cells UACC62 and UACC257 were irradiated with 8 Gy gamma ray to establish an in vitro model, verifying the impact of radiotherapy on gene expression.Results: The risk score demonstrated significant prognostic value and emerged as an independent prognostic factor. miRNA-mRNA and transcription factor regulatory networks underscored its clinical significance. Four key genes were identified: DUSP1, CXCL13, SLAMF7, and EVI2B. Analysis of single-cell and immunotherapy datasets indicated that these genes enhance immune response and immunotherapy efficacy in melanoma patients. PCR results confirmed that gamma rays increased the expression of these genes in human melanoma cells UACC62 and UACC257.Conclusion: Using a multi-omics approach, we analyzed and validated the impact of RT on the immune landscape of melanoma patients. Our findings highlight the critical role of RT-related genes in predicting SKCM prognosis and guiding personalized therapy strategies, particularly in the context of immunotherapy. These contribute to understanding the role of radiotherapy combined with immunotherapy in melanoma.

    Keywords: Skin cutaneous melanoma, Radiotherapy, Immunotherapy, Tumor Microenvironment, prognosis

    Received: 19 Jul 2024; Accepted: 14 Nov 2024.

    Copyright: © 2024 Shi, Zhao, Ding, Ge and Ju. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Mengyang Ju, Osaka University, Suita, 565-0871, Ōsaka, Japan

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.