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ORIGINAL RESEARCH article

Front. Immunol.
Sec. Microbial Immunology
Volume 15 - 2024 | doi: 10.3389/fimmu.2024.1455996

Bacillus amyloliquefaciens TL Promotes Gut Health of Broilers by the Contribution of Bacterial Extracellular Polysaccharides through Its Anti-inflammatory Potential

Provisionally accepted
Shijie Li Shijie Li Pinpin Chen Pinpin Chen Qiuyuan Li Qiuyuan Li Xu Wang Xu Wang Jintao Peng Jintao Peng Ping Xu Ping Xu Hongxia Ding Hongxia Ding Zutao Zhou Zutao Zhou Deshi Shi Deshi Shi Yuncai Xiao Yuncai Xiao *
  • Huazhong Agricultural University, Wuhan, China

The final, formatted version of the article will be published soon.

    The focal point of probiotic efficacy and a crucial factor influencing poultry cultivation lies in the level of intestinal inflammation. In conventional farming processes, the reduction of intestinal inflammation generally proves advantageous for poultry growth. This study investigated the impact of Bacillus amyloliquefaciens TL (B.A.-TL) on inflammatory factor expression at both tissue and cellular levels, alongside an exploration of main active secondary metabolites. The results demonstrated that broiler feeding with a basal diet containing 4 × 10 9 CFU/kg B.A.-TL markedly enhanced chicken growth performance, concomitant with a significant decrease in the expression of genes encoding inflammatory cytokines (e.g., CCL4, CCR5, XCL1, IL-1β, IL-6, IL-8, LITAF, and LYZ) in jejunum and ileum tissues. The extracellular polysaccharides of B.A.-TL (EPS-TL) exhibited notable suppression of elevated inflammatory cytokine expression induced by Escherichia coli O55 lipopolysaccharides (LPS) in chicken macrophage-like cells (HD11) and primary chicken embryonic small intestinal epithelial cells (PCIECs). Moreover, EPS-TL demonstrated inhibitory effect on NF-κB signaling pathway activation. These findings suggested that the metabolic product of B.A.-TL (i.e., EPS-TL) could partly mitigate the enhanced expression of inflammatory factors induced by LPS stimulation, indicating its potential as a key component contributing to the antiinflammatory effects of B.A.-TL.

    Keywords: Bacillus amyloliquefaciens TL, Broiler intestine, Inflammatory factors, Extracellular Polysaccharides, anti-inflammatory activity, NF-κB signaling pathway

    Received: 27 Jun 2024; Accepted: 30 Aug 2024.

    Copyright: © 2024 Li, Chen, Li, Wang, Peng, Xu, Ding, Zhou, Shi and Xiao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Yuncai Xiao, Huazhong Agricultural University, Wuhan, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.