Skip to main content

ORIGINAL RESEARCH article

Front. Immunol.
Sec. Cancer Immunity and Immunotherapy
Volume 15 - 2024 | doi: 10.3389/fimmu.2024.1453220
This article is part of the Research Topic Application of Multi-omics Analysis in Thoracic Cancer Immunotherapy View all 12 articles

Multi-Omics Profiling and Experimental Verification of Tertiary Lymphoid Structure-Related Genes: Molecular Subgroups, Immune Infiltration, and Prognostic Implications in Lung Adenocarcinoma

Provisionally accepted
Wu Sixuan Wu Sixuan 1Junfan Pan Junfan Pan 2*Qihong Pan Qihong Pan 2*Lijun Zeng Lijun Zeng 1*Renji Liang Renji Liang 1*Yuehua Li Yuehua Li 1*
  • 1 The First Affiliated Hospital, University of South China, Hengyang, China
  • 2 Fujian Medical University, Fuzhou, Fujian Province, China

The final, formatted version of the article will be published soon.

    Lung adenocarcinoma (LUAD), characterized by a low 5-year survival rate, is the most common and aggressive type of lung cancer. Recent studies have shown that tertiary lymphoid structures (TLS), which resemble lymphoid structures, are closely linked to the immune response and tumor prognosis.The functions of the tertiary lymphoid structure-related genes (TLS-RGs) in the tumor microenvironment (TME) are poorly understood. Based on publicly available data, we conducted a comprehensive study of the function of TLS-RGs in LUAD. Initially, we categorized LUAD patients into two TLS and two gene subtypes. Subsequently, risk scores were calculated, and prognostic models were constructed using seven genes (CIITA, FCRL2, GBP1, BIRC3, SCGB1A1, CLDN18, and S100P). To enhance the clinical application of TLS scores, we have developed a precise nomogram. Furthermore, drug sensitivity, tumor mutational burden (TMB), and the cancer stem cell (CSC) index were found to be substantially correlated with the TLS scores. Single-cell sequencing results reflected the distribution of TLS-RGs in cells. Finally, we took the intersection of overall survival (OS), disease-specific survival (DSS), and progression-free interval (PFI) prognosis-related genes and then further validated the expression of these genes by qRT-PCR. Our in-depth investigation of TLS-RGs in LUAD revealed their possible contributions to the clinicopathological features, prognosis, and characteristics of TME. These findings underscore the potential of TLS-RGs as prognostic biomarkers and therapeutic targets for LUAD, thereby paving the way for personalized treatment strategies.

    Keywords: Lung Adenocarcinoma, tertiary lymphoid structures, Tumor Microenvironment, Tumor mutation burden, overall survival

    Received: 22 Jun 2024; Accepted: 02 Sep 2024.

    Copyright: © 2024 Sixuan, Pan, Pan, Zeng, Liang and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Junfan Pan, Fujian Medical University, Fuzhou, 350108, Fujian Province, China
    Qihong Pan, Fujian Medical University, Fuzhou, 350108, Fujian Province, China
    Lijun Zeng, The First Affiliated Hospital, University of South China, Hengyang, China
    Renji Liang, The First Affiliated Hospital, University of South China, Hengyang, China
    Yuehua Li, The First Affiliated Hospital, University of South China, Hengyang, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.