AUTHOR=Pan Jin-Min , Liang Yu , Zhu Ke-Cheng , Guo Hua-Yang , Liu Bao-Suo , Zhang Nan , Xian Lin , Zhu Teng-Fei , Zhang Dian-Chang 

TITLE=Genome-wide characterization, phylogenetic and expression analysis of Galectin gene family in Golden pompano Trachinotus ovatus

JOURNAL=Frontiers in Immunology

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2024.1452609

DOI=10.3389/fimmu.2024.1452609

ISSN=1664-3224

ABSTRACT=<p>Galectins (Gals) are a type of S-type lectin that are widespread and evolutionarily conserved among metazoans, and can act as pattern recognition receptors (PRRs) to recognize pathogen-associated molecular patterns (PAMPs). In this study, <italic>10 Gals</italic> (<italic>ToGals</italic>) were identified in the Golden pompano (<italic>Trachinotus ovatus</italic>), and their conserved domains, motifs, and collinearity relationships were analyzed. The expression of <italic>ToGals</italic> was regulated following infection to <italic>Cryptocaryon irritans</italic> and <italic>Streptococcus agalactiae</italic>, indicating that <italic>ToGals</italic> participate in immune responses against microbial pathogens. Further analysis was conducted on one important member, Galectin-3, subcellular localization showing that ToGal-3like protein is expressed both in the nucleus and cytoplasm. Recombinant protein obtained through prokaryotic expression showed that rToGal-3like can agglutinate red blood cells of rabbit, carp and golden pompano and also agglutinate and kill <italic>Staphylococcus aureus, Bacillus subtilis, Vibrio vulnificus, S. agalactiae, Pseudomonas aeruginosa</italic>, and <italic>Aeromonas hydrophila</italic>. This study lays the foundation for further research on the immune roles of <italic>Gals</italic> in teleosts.</p>