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ORIGINAL RESEARCH article

Front. Immunol.
Sec. Comparative Immunology
Volume 15 - 2024 | doi: 10.3389/fimmu.2024.1448627

Age-Specific Changes in Serum Proteome in Female Anadromous, HilsaHilsa Tenualosa iIlisha, (Hamilton, 1822): A Comparative Approach for Understanding Its Variation in Different Stages

Provisionally accepted
Hena Chakraborty Hena Chakraborty 1Hirak J. Chakraborty Hirak J. Chakraborty 1Basanta K. Das Basanta K. Das 1*Joydev Maity Joydev Maity 2
  • 1 Central Inland Fisheries Research Institute (ICAR), Kolkata, India
  • 2 Vidyasagar University, Midnapore, West Bengal, India

The final, formatted version of the article will be published soon.

    The investigation of the proteome profile of the female HilsaHilsa (Tenualosa ilisha), a species of great ecological and economic importance, was conducted across a range of age groups in order to comprehend the functional dynamics of serum proteome for the purposes of conservation and aquaculture as well as to sustain the population. Applying advanced LC-MS/MS base proteomics, including bioinformatics analysis of serum proteome levels show different proteins associated with GO terms, and the genes and associated enriched KEGG pathways (e.g., phagosome, mTOR, Apelin signalingbased proteomics data was analysed and submitted the data in proteomeXchange Consortium via PRIDE., including Bbioinformatics analysis of serum proteome also haves been done and it, showed different proteins associated with GO terms, and the genes associated with enriched KEGG pathways (e.g., phagosome, mTOR, Apelin signalling pathways, herpes simplex virus etc.) that are implicated in immune responses. Significant age-dependent differences were found in the expression levels of important immunological proteins, such as those pertaining to cellular defense, inflammatory response. In this study, we annotated 952, 494, 415 and 282 proteins in the year class IV to class I HilsaHilsa and analysed their PPI (protein-protein interaction) networks with respect toconcerning their functional characteristics. It was noticed that from class I to class IV, new proteins were appearing and it wasappeared and were more than three foldthree-fold. Notably, Formatted: Not Superscript/ Subscript year class-I, displayed a lower abundance of proteins compared to year class-IVclass I displayed a lower abundance of proteins compared to year class IV HilsaHilsa, which displayed higher quantities of proteins. This is the first study to report on the analysis of the serum proteome of HilsaHilsa at different developmental stages, and the results can help to improve the comprehension of the mechanisms underlying the different changes in protein enrichment that occur during migration in HilsaHilsa. This analysis also offers important insights into immune systemsthe immune system for the conservation and management of HilsaHilsa.

    Keywords: HilsaHilsa, Proteomics, protein, String, LC-MS/MS, Network analysis, KEGG Font: Italic Formatted: Font: Italic Formatted: Font: Italic

    Received: 13 Jun 2024; Accepted: 28 Aug 2024.

    Copyright: © 2024 Chakraborty, Chakraborty, Das and Maity. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Basanta K. Das, Central Inland Fisheries Research Institute (ICAR), Kolkata, India

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