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ORIGINAL RESEARCH article

Front. Immunol.
Sec. Comparative Immunology
Volume 15 - 2024 | doi: 10.3389/fimmu.2024.1446710

The significance of CD16+ monocytes in the occurrence and development of Chronic Thromboembolic Pulmonary Hypertension: insights from single-cell RNA sequencing

Provisionally accepted
Maohe Chen Maohe Chen 1Qiuxia Wu Qiuxia Wu 1Nan Shao Nan Shao 1Xinyue Lai Xinyue Lai 1Huo Lin Huo Lin 2Min Chen Min Chen 1Yijing Wu Yijing Wu 1Jiafan Chen Jiafan Chen 1Qinghuang Lin Qinghuang Lin 1Jiahui Huang Jiahui Huang 1Xiaoyun Chen Xiaoyun Chen 1Wei Yan Wei Yan 1Shi Chen Shi Chen 3Hongli Li Hongli Li 1Dawen Wu Dawen Wu 1Minxia Yang Minxia Yang 1Chaosheng Deng Chaosheng Deng 1*
  • 1 First Affiliated Hospital of Fujian Medical University, Fuzhou, China
  • 2 Shishi City General Hospital, Quanzhou, Fujian Province, China
  • 3 Wuhan Sixth Hospital, Wuhan, Hubei Province, China

The final, formatted version of the article will be published soon.

    Chronic thromboembolic pulmonary hypertension (CTEPH) is a complex condition with unclear pathogenesis. We used single-cell RNA sequencing to characterize monocytes in CTEPH patients. We found a significant increase in the CD16+ monocyte subset, which had unique transcriptomic features related to cell adhesion, T cell activation, coagulation, and platelet activation. These features suggest a pivotal role in pulmonary artery thrombus formation and remodeling. We also observed pro-inflammatory macrophage subsets in the tissues from CTEPH patients after pulmonary endarterectomy (PEA). These macrophages showed lipid metabolism reprogramming, which may contribute to thrombus persistence and pulmonary artery intimal remodeling. Notably, we identified a subpopulation of macrophages that differentiated from CD16+ monocytes and highly expressed IL‐1β. This subpopulation was associated with disease progression. Our study reveals the involvement of CD16+ monocytes in CTEPH and presents potential therapeutic targets for intervention.

    Keywords: Chronic thromboembolic pulmonary hypertension, chronic thromboembolic pulmonary disease, Single Cell RNA sequencing, CD16+ monocytes, Macrophages, pulmonary hypertension

    Received: 10 Jun 2024; Accepted: 17 Jul 2024.

    Copyright: © 2024 Chen, Wu, Shao, Lai, Lin, Chen, Wu, Chen, Lin, Huang, Chen, Yan, Chen, Li, Wu, Yang and Deng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Chaosheng Deng, First Affiliated Hospital of Fujian Medical University, Fuzhou, China

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