AUTHOR=Zhou Xiaoli , Song Xizi , Shu Ting , Zhang Silu , Zhang Zhizhu , Hu Canying , Pan Jie , Dai Xiaoshuang , Hao Huaijie , Xiao Guoxun , Wang Pengfei , Liu Kai 

TITLE=Prevention and alleviation of allergic rhinitis by oral administration of Lacticaseibacillus paracasei GOLDGUT-Lpc969

JOURNAL=Frontiers in Immunology

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2024.1444778

DOI=10.3389/fimmu.2024.1444778

ISSN=1664-3224

ABSTRACT=<sec><title>Introduction</title><p>Allergic rhinitis (AR) is a widespread upper airway disorder characterized by inflammation of the nasal passages. It is immunologically mediated via the hypersensitivity type I mechanism, which is primarily elicited by the immunoglobulin E (IgE)-linking allergen-induced imbalance of the Th2/Th1 immune response. Owing to the limited efficacy of current medications, probiotics have received attention for their potential in preventing and ameliorating AR.</p></sec><sec><title>Methods</title><p>In this study, a <italic>Lacticaseibacillus</italic> paracasei strain, GOLDGUTLpc969 (Lpc969), isolated from the feces of healthy adults, was proven to be effective in preventing AR by LPA-induced RBL-2H3 <italic>in-vitro</italic> and OVA-induced AR mice <italic>in-vivo</italic> evaluation.</p></sec><sec><title>Results</title><p>The strain significantly attenuated the release of histamine and degranulation in LPS-induced RBL-2H3 cells. In the OVA-induced AR mice, <italic>L. paracase</italic>i GOLDGUT-Lpc969 also exhibited a significant decrease in disease indicators such as the disease activity index (DAI score), serum IgE, and serum histamine. Treatment with <italic>L. paracasei</italic> GOLDGUT-Lpc969 led to significant suppression of the Th2-related cytokines IL-4, IL-5, IL-6, IL-13, and TNF-α in the serum of mice.</p></sec><sec><title>Discussion</title><p>Furthermore, a comparison of the genomes of three previously reported AR-effective <italic>L. paracasei</italic> strains (including GOLDGUTLpc969) and one non-effective <italic>L. paracasei</italic> strain revealed that the gene K03671 may play a key role in alleviating AR symptoms. In conclusion, this study highlights the efficacy of <italic>L. paracasei</italic> GOLDGUT-Lpc969 in AR prevention by suppressing the Th2 immune response and proposes the potential involvement of the functional gene K03671 in ameliorating AR symptoms. Therefore, <italic>L. paracasei</italic> GOLDGUT-Lpc969 shows promise as a probiotic for preventing AR.</p></sec>