AUTHOR=Hartmann Carina R. , Khan Robin , Schöning Jennifer , Richter Maximilian , Willers Maike , Pirr Sabine , Heckmann Julia , Dirks Johannes , Morbach Henner , Konrad Monika , Fries Elena , Winkler Magdalene , Büchel Johanna , Seidenspinner Silvia , Fischer Jonas , Vollmuth Claudia , Meinhardt Martin , Marissen Janina , Schmolke Mirco , Haid Sibylle , Pietschmann Thomas , Backes Simone , Dölken Lars , Löber Ulrike , Keil Thomas , Heuschmann Peter U. , Wöckel Achim , Sagar , Ulas Thomas , Forslund-Startceva Sofia K. , Härtel Christoph , Viemann Dorothee TITLE=A clinical protocol for a German birth cohort study of the Maturation of Immunity Against respiratory viral Infections (MIAI) JOURNAL=Frontiers in Immunology VOLUME=15 YEAR=2024 URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2024.1443665 DOI=10.3389/fimmu.2024.1443665 ISSN=1664-3224 ABSTRACT=Introduction

Respiratory viral infections (RVIs) are a major global contributor to morbidity and mortality. The susceptibility and outcome of RVIs are strongly age-dependent and show considerable inter-population differences, pointing to genetically and/or environmentally driven developmental variability. The factors determining the age-dependency and shaping the age-related changes of human anti-RVI immunity after birth are still elusive.

Methods

We are conducting a prospective birth cohort study aiming at identifying endogenous and environmental factors associated with the susceptibility to RVIs and their impact on cellular and humoral immune responses against the influenza A virus (IAV), respiratory syncytial virus (RSV) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The MIAI birth cohort enrolls healthy, full-term neonates born at the University Hospital Würzburg, Germany, with follow-up at four defined time-points during the first year of life. At each study visit, clinical metadata including diet, lifestyle, sociodemographic information, and physical examinations, are collected along with extensive biomaterial sampling. Biomaterials are used to generate comprehensive, integrated multi-omics datasets including transcriptomic, epigenomic, proteomic, metabolomic and microbiomic methods.

Discussion

The results are expected to capture a holistic picture of the variability of immune trajectories with a focus on cellular and humoral key players involved in the defense of RVIs and the impact of host and environmental factors thereon. Thereby, MIAI aims at providing insights that allow unraveling molecular mechanisms that can be targeted to promote the development of competent anti-RVI immunity in early life and prevent severe RVIs.

Clinical trial registration

https://drks.de/search/de/trial/, identifier DRKS00034278.