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ORIGINAL RESEARCH article
Front. Immunol.
Sec. Cancer Immunity and Immunotherapy
Volume 15 - 2024 |
doi: 10.3389/fimmu.2024.1438935
This article is part of the Research Topic Harnessing Single-Cell Insights: Pioneering Predictive Markers for Immunotherapy Efficacy in Solid Tumors View all 7 articles
Role of Glycosylation-Related Gene MGAT1 in Pancreatic Ductal Adenocarcinoma
Provisionally accepted
Lai Jiang
1
Jie Liu
2,3*
Shengke Zhang
1
Chenglu Jiang
1
Jinbang Huang
1
Haiqing Chen
1
Xuancheng Zhou
1
Yiping Fu
1*
Zhongqiu Yang
2*
Rui Wang
3*
Guanhu Yang
4*
Hao Chi
1*
Bo Li
3*- 1 Southwest Medical University, Luzhou, China
- 2 Dazhou Central Hospital, DaZhou, Sichuan Province, China
- 3 The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, China
- 4 Ohio University, Athens, West Virginia, United States
Background: pancreatic ductal adenocarcinoma (PDAC) is a malignant tumor with a very poor prognosis and a complex tumor microenvironment, which plays a key role in tumor progression and treatment resistance. Glycosylation plays an important role in processes such as cell signaling, immune response and protein stability. Materials and methods: single-cell RNA sequencing data and spatial transcriptome data were obtained from GSE197177 and GSE224411, respectively, and RNA-seq data and survival information were obtained from UCSC Xena and TCGA. Multiple transcriptomic data were comprehensively analysed to explore the role of glycosylation processes in tumor progression, and functional experiments were performed to assess the effects of MGAT1 overexpression on PDAC cell proliferation and migration. Results: In PDAC tumor samples, the glycosylation level of macrophages was significantly higher than that of normal samples. MGAT1 was identified as a key glycosylation-related gene, and its high expression was associated with better patient prognosis. Overexpression of MGAT1 significantly inhibited the proliferation and migration of PDAC cells and affected intercellular interactions in the tumor microenvironment. Conclusion: MGAT1 plays an important role in PDAC by regulating glycosylation levels in macrophages, influencing tumor progression and improving prognosis.MGAT1 is a potential therapeutic target for PDAC and further studies are needed to develop targeted therapeutic strategies against MGAT1 to improve clinical outcomes.
Keywords: Single-Cell Analysis, Immunotherapy, predictive marker, Glycosylation, glycosyltransferase, Macrophages
Received: 27 May 2024; Accepted: 18 Jul 2024.
Copyright: © 2024 Jiang, Liu, Zhang, Jiang, Huang, Chen, Zhou, Fu, Yang, Wang, Yang, Chi and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Jie Liu, Dazhou Central Hospital, DaZhou, Sichuan Province, China
Yiping Fu, Southwest Medical University, Luzhou, China
Zhongqiu Yang, Dazhou Central Hospital, DaZhou, Sichuan Province, China
Rui Wang, The Affiliated Hospital of Southwest Medical University, Luzhou, 646000, Sichuan, China
Guanhu Yang, Ohio University, Athens, 45701, West Virginia, United States
Hao Chi, Southwest Medical University, Luzhou, China
Bo Li, The Affiliated Hospital of Southwest Medical University, Luzhou, 646000, Sichuan, China
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