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ORIGINAL RESEARCH article

Front. Immunol.
Sec. Microbial Immunology
Volume 15 - 2024 | doi: 10.3389/fimmu.2024.1435151
This article is part of the Research Topic Advances in Drug Delivery Systems to Combat Multidrug Resistant Bacteria View all articles

Antibiotics-encapsulated nanoparticles as an antimicrobial agent in the treatment of wound infection

Provisionally accepted
Mohammad Taheri Mohammad Taheri 1Mohammad R. Arabestani Mohammad R. Arabestani 1Fereshte Kalhori Fereshte Kalhori 1Sara Soleimani Asl Sara Soleimani Asl 1Masoumeh Asgari Masoumeh Asgari 1Seyed Mostafa Hosseini Seyed Mostafa Hosseini 1,2*
  • 1 Hamadan University of Medical Sciences, Hamedan, Hamadan, Iran
  • 2 Department of Microbiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

The final, formatted version of the article will be published soon.

    Disruption in the wound-healing process is caused by the presence of bacteria and leads to major problems and delays in wound healing. The limitations of commonly used medicines for treating wound infections include drug toxicity, insufficient microbial coverage, poor penetration, and increased resistance. This study aimed to determine the effect of ciprofloxacin loaded in solid lipid nanoparticles (Cip-SLN) on Pseudomonas aeruginosa and ampiciliin-vancomycin loaded in solid lipid nanoparticles (Amp- Van-SLN) on Staphylococcus aureus in wounds. Antibiotics were encapsulated in SLNs using the double emulsion method and were characterized. The in-vitro effect of antibiotic-loaded nanoparticles on P. aeruginosa and S. aureus was assessed using well diffusion and MIC methods. Finally, the topical antibacterial activity of these nanoparticles against bacterial wound infection was measured in a mouse model. MIC results showed that in the first 24 hours, the free drug had a greater effect on inhibiting bacteria, and in 72 hours, the inhibitory effect of nanoparticles increased. There was no toxicity effect of 400 µg/mL of nanoparticles on cells. According to the findings, the groups treated with Cip-SLN and Amp-Van-SLN were more effective than the control group (untreated) in different concentrations. In the wound healing process, the group treated with solid lipid nanoparticles (SLNs) exhibited a greater epithelial thickness, indicating enhanced healing, compared to the group treated with the free drug. The use of SLN can increase the accumulation of antibiotics at the site of infection with a slow release of the drug due to its fatty nature, which leads to a significant inhibitory effect on bacteria and also improves wound healing.

    Keywords: Wound Healing, Ciprofloxacin, Vancomycin, Solid lipid nanoparticles, Pseudomonas aeruginosa, Staphylococcus aureus

    Received: 19 May 2024; Accepted: 08 Oct 2024.

    Copyright: © 2024 Taheri, Arabestani, Kalhori, Soleimani Asl, Asgari and Hosseini. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Seyed Mostafa Hosseini, Department of Microbiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.