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ORIGINAL RESEARCH article

Front. Immunol.
Sec. Immunological Tolerance and Regulation
Volume 15 - 2024 | doi: 10.3389/fimmu.2024.1431303
This article is part of the Research Topic Exploring the Molecular Mechanisms of Immune Related Diseases Through Multi-Omics Studies View all articles

Single-cell multi-omics reveal stage of differentiation and trajectorydependent immunity-related gene expression patterns in human erythroid cells

Provisionally accepted
Roman Perik-Zavodskii Roman Perik-Zavodskii 1Olga Perik-Zavodskaia Olga Perik-Zavodskaia 1Saleh Alrhmoun Saleh Alrhmoun 1Marina Volynets Marina Volynets 1Julia Shevchenko Julia Shevchenko 1Kirill Nazarov Kirill Nazarov 1Vera Denisova Vera Denisova 2Sergey V. Sennikov Sergey V. Sennikov 1*
  • 1 Research Institute for Fundamental and Clinical Immunology (NIIFKI), Novosibirsk, Russia
  • 2 Clinic of Immunopathology, Research Institute for Fundamental and Clinical Immunology (NIIFKI), Novosibirsk, Novosibirsk Oblast, Russia

The final, formatted version of the article will be published soon.

    The role of Erythroid cells in immune regulation and immunosuppression is one of the emerging topics in modern immunology that still requires further clarification as Erythroid cells from different tissues and different species express different immunoregulatory molecules. In this study, we performed a thorough investigation of human bone marrow Erythroid cells from adult healthy donors and adult acute lymphoblastic leukemia patients using the state-of-the-art single-cell targeted proteomics and transcriptomics via BD Rhapsody and cancer-related gene copy number variation analysis via NanoString Sprint Profiler. We found that human bone marrow Erythroid cells express the ARG1, LGALS1, LGALS3, LGALS9, and C10orf54 (VISTA) immunosuppressive genes, CXCL5, CXCL8, and VEGFA cytokine genes, as well as the genes involved in antimicrobial immunity and MHC Class II antigen presentation. We also found that ARG1 gene expression was restricted to the single erythroid cell cluster that we termed ARG1-positive Orthochromatic erythroblasts and that late Erythroid cells lose S100A9 and gain MZB1 gene expression in case of acute lymphoblastic leukemia. These findings show that steady-state erythropoiesis bone marrow Erythroid cells express myeloid signature genes even without any transdifferentiating stimulus like cancer.

    Keywords: Erythroid Cells, CD71+ erythroid cells, CECs, Acute Lymphoblastic Leukemia, ALL, CITE-seq, ScRNA-seq

    Received: 11 May 2024; Accepted: 09 Aug 2024.

    Copyright: © 2024 Perik-Zavodskii, Perik-Zavodskaia, Alrhmoun, Volynets, Shevchenko, Nazarov, Denisova and Sennikov. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Sergey V. Sennikov, Research Institute for Fundamental and Clinical Immunology (NIIFKI), Novosibirsk, Russia

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.