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ORIGINAL RESEARCH article
Front. Immunol.
Sec. Immunological Tolerance and Regulation
Volume 15 - 2024 |
doi: 10.3389/fimmu.2024.1431175
Peripheral Immune Characteristics and Subset Disorder in Reproductive Females with Endometriosis
Provisionally accepted
Kai-Rong Lin
*
Pei-Xian Li
*
Xiao-Hong Zhu
*
Xiaofan Mao
Jia-Li Peng
*
Xiang-Ping Chen
*
Cui-Yao Situ
*
Li-Fang Zhang
*
Wei Luo
*
Yu-Bin Han
*
Sifei Yu
*- First People's Hospital of Foshan, Foshan, Guangdong Province, China
Pathogenesis of endometriosis (EN) is still unknown, but growing evidence suggests that immune regulation may be important, and the pattern of peripheral immune changes in reproductive women with EN has yet to be fully explored. In this study, we conducted a comprehensive and systematic analysis of immune cell subsets within T cells, B cells, NK cells, and γδ T cells in peripheral blood (PB) samples from women with EN, women with uterine fibroids (UF) but without EN (UF-alone), and healthy controls using multi-parameter flow cytometry. Our findings revealed that UF, a common comorbidity of EN, exhibited similar peripheral immune features to EN, particularly in T cell and B cell immunity. Compared to healthy controls, we constructed the peripheral immune profile of EN. This profile highlighted that the immunopathogenic factors in EN predominantly relate to the immune disorder of B cells and their subsets, as well as the functional abnormalities within immune cell subsets of CD4 + T cells, CD8 + T cells, and γδ T cells. Moreover, using the random forest (RF) machine-learning method, we developed a diagnostic model that can effectively identify the patients with EN from healthy controls. The immune factors identified within this model could be pivotal for unraveling the immune pathogenic mechanisms of EN. Our study is the first to present a comprehensive depiction of the circulating immune features in EN, although the detailed roles and underlying mechanisms of these immune factors in the context of EN require further investigation.
Keywords: Endometriosis, peripheral immune profile, immnue cell subset, Flow Cytometry, diagnosis
Received: 13 May 2024; Accepted: 11 Nov 2024.
Copyright: © 2024 Lin, Li, Zhu, Mao, Peng, Chen, Situ, Zhang, Luo, Han and Yu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Kai-Rong Lin, First People's Hospital of Foshan, Foshan, 528300, Guangdong Province, China
Pei-Xian Li, First People's Hospital of Foshan, Foshan, 528300, Guangdong Province, China
Xiao-Hong Zhu, First People's Hospital of Foshan, Foshan, 528300, Guangdong Province, China
Jia-Li Peng, First People's Hospital of Foshan, Foshan, 528300, Guangdong Province, China
Xiang-Ping Chen, First People's Hospital of Foshan, Foshan, 528300, Guangdong Province, China
Cui-Yao Situ, First People's Hospital of Foshan, Foshan, 528300, Guangdong Province, China
Li-Fang Zhang, First People's Hospital of Foshan, Foshan, 528300, Guangdong Province, China
Wei Luo, First People's Hospital of Foshan, Foshan, 528300, Guangdong Province, China
Yu-Bin Han, First People's Hospital of Foshan, Foshan, 528300, Guangdong Province, China
Sifei Yu, First People's Hospital of Foshan, Foshan, 528300, Guangdong Province, China
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