Skip to main content

ORIGINAL RESEARCH article

Front. Immunol.
Sec. Autoimmune and Autoinflammatory Disorders : Autoimmune Disorders
Volume 15 - 2024 | doi: 10.3389/fimmu.2024.1406445

TMEM106A: A new biomarker of atherosclerosis

Provisionally accepted
Menglong Gao Menglong Gao *Min Dai Min Dai *Yarong Liu Yarong Liu *
  • Anhui University of Chinese Medicine, Hefei, Anhui Province, China

The final, formatted version of the article will be published soon.

    The interplay between atherosclerosis (AS) and immunity is complex, yet research on AS biomarkers is currently lacking, highlighting the necessity for novel biomarker discovery.We used machine learning algorithms to identify marker genes associated with AS using the GSE43292 dataset. We further validated the differential expression of these marker genes across multiple datasets. Enrichment analysis was conducted to explore the functional pathways related to these biomarkers. We employed methods to quantify immune cell infiltration and identify relevant immune cells. In addition, we observed the expression of biomarkers in AS plaques on single-cell datasets and through immunofluorescence double staining of ApoE -/-mice.Macrophage immune infiltration was evaluated through ROS fluorescence, flow cytometry, and Elisa detection of inflammatory factors.The biomarker TMEM106A exhibited significant expression differences in AS patients. TMEM106A showed high expression levels specifically in macrophages within AS plaques. Silencing TMEM106A in macrophage RAW264.7 cells resulted in reduced foam cell formation, decreased ROS production, diminished release of inflammatory factors induced by LPS and oxidized LDL.TMEM106A is involved in macrophage-mediated immune infiltration in AS. TMEM106A may contribute to AS progression by promoting macrophage infiltration.

    Keywords: Atherosclerosis, biomarkers, TMEM106a, Immunity, Macrophages

    Received: 25 Mar 2024; Accepted: 14 Oct 2024.

    Copyright: © 2024 Gao, Dai and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Menglong Gao, Anhui University of Chinese Medicine, Hefei, 230038, Anhui Province, China
    Min Dai, Anhui University of Chinese Medicine, Hefei, 230038, Anhui Province, China
    Yarong Liu, Anhui University of Chinese Medicine, Hefei, 230038, Anhui Province, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.