This article is a correction to:
Sulforaphane diminishes moonlighting of pyruvate kinase M2 and interleukin 1β expression in M1 (LPS) macrophages
Sheyda Bahiraii
Martin Brenner
Fangfang Yan
Wolfram Weckwerth
Elke H. Heiss- 1Department of Pharmaceutical Sciences, University of Vienna, Vienna, Austria
- 2Vienna Doctoral School of Pharmaceutical, Nutritional and Sport Sciences (VDS PhaNuSpo), University of Vienna, Vienna, Austria
- 3Vienna Metabolomics Center (VIME), University of Vienna, Vienna, Austria
- 4College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China
- 5Molecular Systems Biology (MOSYS), Department of Functional and Evolutionary Ecology, University of Vienna, Vienna, Austria
A Corrigendum on
Sulforaphane diminishes moonlighting of pyruvate kinase M2 and interleukin 1β expression in M1 (LPS) macrophages
by Bahiraii S, Brenner M, Yan F, Weckwerth W and Heiss EH (2022). Front. Immunol. 13:935692. doi: 10.3389/fimmu.2022.935692
In the published article, there was an error in the legend for Figure 7, panel B as published. The labeling of the lanes in the immunoblots was incorrect.
Figure 7
Figure 7 Sfn does not affect Y105 phosphorylation but glutathionylation of PKM2 in M1 (LPS) macrophages. iBMDM were pretreated with DMSO or the indicated concentrations of Sfn for 30 min prior to stimulation with LPS for 6 h. (A) Total cell lysates were immunoblotted for p(Y105), total PKM2, and actin as a loading control. Representative blots of at least three independent experiments are depicted. Bar graphs represent compiled densitometric data. (B) Macrophages were pretreated with Sfn (10 μM) or the GSH donor glutathioneethylester (GSEE) before LPS was added for another 6 h. Proteins were harvested under nonreducing conditions. Lysates (input) or immunoprecipitates obtained with an antibody specific for protein-bound glutathione (GSS-X) were subjected to immunoblot analysis for PKM2. Representative blots of three independent experiments with consistent results are depicted.
The corrected legend appears below.
“Sfn does not affect Y105 phosphorylation but glutathionylation of PKM2 in M1 (LPS) macrophages. iBMDM were pretreated with DMSO or the indicated concentrations of Sfn for 30 min prior to stimulation with LPS for 6 h. (A) Total cell lysates were immunoblotted for p(Y105), total PKM2, and actin as a loading control. Representative blots of at least three independent experiments are depicted. Bar graphs represent compiled densitometric data. (B) Macrophages were pretreated with Sfn (10 µM) or the GSH donor glutathione-ethylester (GSEE) before LPS was added for another 6 h. Proteins were harvested under nonreducing conditions. Lysates (input) or immunoprecipitates obtained with an antibody specific for protein-bound glutathione (GSS-X) were subjected to immunoblot analysis for PKM2. Representative blots of three independent experiments with consistent results are depicted.”
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
In the published article, there was an error in Figure 7, panel B as published. The labeling of the lanes in the immunoblots was incorrect. The corrected Figure 7 appears below.
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
Publisher’s note
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Keywords: sulforaphane, macrophages, M1 polarization, glycolysis, PKM2, interleukin 1 beta
Citation: Bahiraii S, Brenner M, Yan F, Weckwerth W and Heiss EH (2024) Corrigendum: Sulforaphane diminishes moonlighting of pyruvate kinase M2 and interleukin 1β expression in M1 (LPS) macrophages. Front. Immunol. 15:1395642. doi: 10.3389/fimmu.2024.1395642
Received: 04 March 2024; Accepted: 09 April 2024;
Published: 22 April 2024.
Edited and Reviewed by:
Francesca Granucci, University of Milano-Bicocca, ItalyCopyright © 2024 Bahiraii, Brenner, Yan, Weckwerth and Heiss. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Elke H. Heiss, elke.heiss@univie.ac.at