AUTHOR=Shen Man , Dai Xianling , Ning Dongni , Xu Hanqing , Zhou Yang , Chen Gangan , Ren Zhangyin , Chen Ming , Gao Mingxuan , Bao Jing 

TITLE=Integrating Pt nanoparticles with 3D Cu2-xSe/GO nanostructure to achieve nir-enhanced peroxidizing Nano-enzymes for dynamic monitoring the level of H2O2 during the inflammation

JOURNAL=Frontiers in Immunology

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2024.1392259

DOI=10.3389/fimmu.2024.1392259

ISSN=1664-3224

ABSTRACT=<p>The treatment of wound inflammation is intricately linked to the concentration of reactive oxygen species (ROS) in the wound microenvironment. Among these ROS, H<sub>2</sub>O<sub>2</sub> serves as a critical signaling molecule and second messenger, necessitating the urgent need for its rapid real-time quantitative detection, as well as effective clearance, in the pursuit of effective wound inflammation treatment. Here, we exploited a sophisticated 3D Cu<sub>2-</sub><italic><sub>x</sub></italic>Se/GO nanostructure-based nanonzymatic H<sub>2</sub>O<sub>2</sub> electrochemical sensor, which is further decorated with evenly distributed Pt nanoparticles (Pt NPs) through electrodeposition. The obtained Cu<sub>2-</sub><italic><sub>x</sub></italic>Se/GO@Pt/SPCE sensing electrode possesses a remarkable increase in specific surface derived from the three-dimensional surface constructed by GO nanosheets. Moreover, the localized surface plasma effect of the Cu<sub>2-</sub><italic><sub>x</sub></italic>Se nanospheres enhances the separation of photogenerated electron-hole pairs between the interface of the Cu<sub>2-</sub><italic><sub>x</sub></italic>Se NPs and the Pt NPs. This innovation enables near-infrared light-enhanced catalysis, significantly reducing the detection limit of the Cu<sub>2-</sub><italic><sub>x</sub></italic>Se/GO@Pt/SPCE sensing electrode for H<sub>2</sub>O<sub>2</sub> (from 1.45 μM to 0.53μM) under NIR light. Furthermore, this biosensor electrode enables <italic>in-situ</italic> real-time monitoring of H<sub>2</sub>O<sub>2</sub> released by cells. The NIR-enhanced Cu<sub>2-</sub><italic><sub>x</sub></italic>Se/GO@Pt/SPCE sensing electrode provide a simple-yet-effective method to achieve a detection of ROS (H<sub>2</sub>O<sub>2</sub>、-OH) with high sensitivity and efficiency. This innovation promises to revolutionize the field of wound inflammation treatment by providing clinicians with a powerful tool for accurate and rapid assessment of ROS levels, ultimately leading to improved patient outcomes.</p>