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ORIGINAL RESEARCH article
Front. Immunol.
Sec. Inflammation
Volume 15 - 2024 |
doi: 10.3389/fimmu.2024.1375781
Huang Lian Jie Du decoction attenuated colitis via suppressing macrophage Csf1r/Src pathway and modulating gut microbiota
Provisionally accepted- 1 The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, China
- 2 Department of Neurology, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China, Guangzhou, China
- 3 Department of Neurology, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, China
- 4 Department of Pharmacy, Shenzhen Children’s Hospital, Shenzhen, China, Shenzhen, China
- 5 Department of Food Science and Nutrition, The Hong Kong Polytechnic University, Hong Kong, China
- 6 Department of Applied Biology & Chemical Technology, Faculty of Science, Hong Kong Polytechnic University, Kowloon, Hong Kong, SAR China
- 7 State Key Laboratory of Chemical Biology and Drug Discovery, Hong Kong Polytechnic University, Hong Kong, China
- 8 Research Centre for Chinese Medicine Innovation, Hong Kong Polytechnic University, Kowloon, Hong Kong, SAR China
- 9 State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Shenzhen Research Institute, Hong Kong Polytechnic University, Shenzhen, China
- 10 Shenzhen Research Institute, Hong Kong Polytechnic University, Shenzhen, China
Ulcerative colitis, a subtype of chronic inflammatory bowel disease (IBD), is characterized by relapsing colonic inflammation and ulcers. Traditional Chinese herbal formulation Huang Lian Jie Du (HLJD) decoction is used clinically to treat diarrhea and colitis. However, the mechanism(s) associated with the effects of treatment remains unclear. This study aims to elucidate the molecular mechanistic effects of HLJD formulation on colitis. Chronic colitis in mice were induced by 1% dextran sulfate sodium (DSS) in drinking water continuously for 8 weeks, and HLJD decoction at the doses of 2 g/kg and 4 g/kg were administered orally to mice daily from the second week until experimental endpoint. Stool consistency scores, blood stool scores, and body weights were recorded weekly. Disease activity index (DAI) was determined before necropsy, where colon tissues were collected for biochemical analyses. In addition, the fecal microbiome of treated mice was characterized using 16S rRNA amplicon sequencing. HLJD decoction at doses of 2 g/kg and 4 g/kg relieved DSS-induced chronic colitis in mice by suppressing inflammation through compromised macrophage activity in colonic tissues associated with the colony stimulating factor 1 receptor (Csf1r)/Src pathway. Furthermore, HLJD formula could modify the gut microbiota profile by decreasing the abundance of Bacteroides, Odoribacter, Clostridium_sensu_stricto_1 and Parasutterella. In addition, close correlations between DAI, colon length, spleen weight and gut microbiota were identified. Our findings revealed that HLJD formula attenuated DSS-induced chronic colitis by reducing inflammation via Csf1r/Src mediated macrophage infiltration, as well as modulating the gut microbiota profile.
Keywords: Colitis, macrophage, Csf1r, src, Gut Microbiota
Received: 24 Jan 2024; Accepted: 30 Aug 2024.
Copyright: © 2024 Su, Liu, Zheng, Wu, Keng, Zhang and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Vincent W. Keng, Department of Applied Biology & Chemical Technology, Faculty of Science, Hong Kong Polytechnic University, Kowloon, Hong Kong, SAR China
Shi-Jie Zhang, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, China
Xiaoxiao Li, Shenzhen Research Institute, Hong Kong Polytechnic University, Shenzhen, China
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