AUTHOR=Su Rui , Kang Xue , Niu Yifan , Zhao Tiesuo , Wang Hui TITLE=PCBP1 interacts with the HTLV-1 Tax oncoprotein to potentiate NF-κB activation JOURNAL=Frontiers in Immunology VOLUME=15 YEAR=2024 URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2024.1375168 DOI=10.3389/fimmu.2024.1375168 ISSN=1664-3224 ABSTRACT=

Human T-cell leukemia virus type 1 (HTLV-1) is the etiological agent of adult T-cell leukemia/lymphoma. The HTLV-1 Tax constitutively activates nuclear factor-κB (NF-κB) to promote the survival and transformation of HTLV-1-infected T cells. Despite extensive study of Tax, how Tax interacts with host factors to regulate NF-κB activation and HTLV-1–driven cell proliferation is not entirely clear. Here, we showed that overexpression of Poly (rC)–binding protein 1 (PCBP1) promoted Tax-mediated IκB kinase (IKK)–NF-κB signaling activation, whereas knockdown of PCBP1 attenuated Tax-dependent IKK–NF-κB activation. However, Tax activation of HTLV-1 long terminal repeat was unaffected by PCBP1. Furthermore, depletion of PCBP1 led to apoptosis and reduced proliferation of HTLV-1–transformed cells. Mechanistically, PCBP1 interacted and co-localized with Tax in the cytoplasm, and PCBP1 KH3 domain was indispensable for the interaction between PCBP1 and Tax. Moreover, PCBP1 facilitated the assembly of Tax/IKK complex. Collectively, our results demonstrated that PCBP1 may exert an essential effect in Tax/IKK complex combination and subsequent NF-κB activation, which provides a novel insight into the pathogenetic mechanisms of HTLV-1.