AUTHOR=Peng Xue Yun , Wang Kai Lun , Li Li , Li Bo , Wu Xiang Yang , Zhang Zhi Wei , Li Nan , Liu Lan Hao , Nie P. , Chen Shan Nan 

TITLE=Transcription of NOD1 and NOD2 and their interaction with CARD9 and RIPK2 in IFN signaling in a perciform fish, the Chinese perch, Siniperca chuatsi

JOURNAL=Frontiers in Immunology

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2024.1374368

DOI=10.3389/fimmu.2024.1374368

ISSN=1664-3224

ABSTRACT=<p>NOD1 and NOD2 as two representative members of nucleotide-binding oligomerization domain (NOD)-like receptor (NLR) family play important roles in antimicrobial immunity. However, transcription mechanism of <italic>nod1</italic> and <italic>nod2</italic> and their signal circle are less understood in teleost fish. In this study, with the cloning of <italic>card9</italic> and <italic>ripk2</italic> in Chinese perch, the interaction between NOD1, NOD2, and CARD9 and RIPK2 were revealed through coimmunoprecipitation and immunofluorescence assays. The overexpression of NOD1, NOD2, RIPK2 and CARD9 induced significantly the promoter activity of NF-κB, IFNh and IFNc. Furthermore, it was found that <italic>nod1</italic> and <italic>nod2</italic> were induced by poly(I:C), type I IFNs, RLR and even NOD1/NOD2 themselves through the ISRE site of their proximal promoters. It is thus indicated that <italic>nod1</italic> and <italic>nod2</italic> can be classified also as ISGs due to the presence of ISRE in their proximal promoter, and their expression can be mechanistically controlled through PRR pathway as well as through IFN signaling in antiviral immune response.</p>