AUTHOR=Zhang Xin , Ma Sisi , Huebner Janet L. , Naz Syeda Iffat , Alnemer Noor , Soderblom Erik J. , Aliferis Constantin , Kraus Virginia Byers 

TITLE=Immune system-related plasma extracellular vesicles in healthy aging

JOURNAL=Frontiers in Immunology

VOLUME=Volume 15 - 2024

YEAR=2024

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2024.1355380

DOI=10.3389/fimmu.2024.1355380

ISSN=1664-3224

ABSTRACT=Objectives: To identify age-related plasma extracellular vehicle (EVs) phenotypes in healthy adults.Methods: EV proteomics by high-resolution mass spectrometry to evaluate EV protein stability and discover age-associated EV proteins (n=4 with 4 serial freeze-thaws each); validation by highresolution flow cytometry and EV cytokine quantification by multiplex ELISA (n=28 healthy donors, aged 18-83 years); quantification of WI-38 fibroblast cell proliferation response to co-culture with PKH67-labeled young and old plasma EVs. The EV samples from these plasma specimens were previously characterized for bilayer structure, intra-vesicle mitochondria and cytokines, and hematopoietic cell-related surface markers.Results: Compared with matched exo-EVs (EV-depleted supernatants), endo-EVs (EV-associated)