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ORIGINAL RESEARCH article

Front. Immunol.
Sec. Systems Immunology
Volume 15 - 2024 | doi: 10.3389/fimmu.2024.1354926

Altered immune cell in human severe acute pancreatitis revealed by single-cell RNA sequencing

Provisionally accepted
Wu Zheyi Wu Zheyi 1,2*Wang Shijie Wang Shijie 1*Zhiheng Wu Zhiheng Wu 2Tao Junjie Tao Junjie 3*Li Lei Li Lei 4*Zheng Chuanming Zheng Chuanming 3*Xu Zhipeng Xu Zhipeng 4*Du Zhaohui Du Zhaohui 3*Zhao Chengpu Zhao Chengpu 3Liang Pengzhen Liang Pengzhen 1*Xu Aman Xu Aman 5*Wang Zhenjie Wang Zhenjie 1*
  • 1 Department of Emergency Surgery, Department of Emergency Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui Province, China
  • 2 Department of General Surgery, Huangshan City People’s Hospital, Huangshan, Anhui Province, China
  • 3 Department of Emergency Surgery, Department of General Surgery, The First Affiliated Hospital of Bengbu Medical College, Bnegbu, China
  • 4 Department of Emergency Surgery, National Drug Clinical Trial Institute, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui Province, China
  • 5 Department of Gastrointestinal Surgery, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui Province, China

The final, formatted version of the article will be published soon.

    This study explores critical subpopulations and their genetic alterations within peripheral blood mononuclear cells (PBMCs) using single-cell sequencing technology (scRNA-seq) in the context of severe acute pancreatitis (SAP). Initially, the study identifies a total of 20 distinct cell subtypes. Notably, a novel finding in this research is the identification of a specific monocyte subpopulation characterized by elevated expression of PF4 and PPBP.In comparison to healthy controls, SAP patients exhibit lymphocyte depletion, an increase in monocytes, and pronounced activation or upregulation of inflammatory pathways and genes. Particularly, the inflammatory pathways and receptor activation pathways related to monocytes are prominently affected. The study also elucidates the patterns of intercellular communication within these subpopulations.Lastly, Scissor is applied to identify cellular subpopulations associated with disease severity in PBMCs from the SAP single-cell dataset, guided by 87 large-sample acute pancreatitis whole transcriptome datasets with disease severity information. In summary, leveraging scRNA-seq, this study provides valuable insights into the roles and potential mechanisms of different cell subpopulations in SAP. Furthermore, it introduces a set of potential biomarkers with clinical significance for predicting the severity of SAP.

    Keywords: Severe acute pancreatitis, single cell RNA-sequencing, Inflammatory, Monocytes, Transcriptomic analyses

    Received: 19 Dec 2023; Accepted: 03 Sep 2024.

    Copyright: © 2024 Zheyi, Shijie, Wu, Junjie, Lei, Chuanming, Zhipeng, Zhaohui, Chengpu, Pengzhen, Aman and Zhenjie. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Wu Zheyi, Department of Emergency Surgery, Department of Emergency Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui Province, China
    Wang Shijie, Department of Emergency Surgery, Department of Emergency Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui Province, China
    Tao Junjie, Department of Emergency Surgery, Department of General Surgery, The First Affiliated Hospital of Bengbu Medical College, Bnegbu, China
    Li Lei, Department of Emergency Surgery, National Drug Clinical Trial Institute, The First Affiliated Hospital of Bengbu Medical College, Bengbu, 233004, Anhui Province, China
    Zheng Chuanming, Department of Emergency Surgery, Department of General Surgery, The First Affiliated Hospital of Bengbu Medical College, Bnegbu, China
    Xu Zhipeng, Department of Emergency Surgery, National Drug Clinical Trial Institute, The First Affiliated Hospital of Bengbu Medical College, Bengbu, 233004, Anhui Province, China
    Du Zhaohui, Department of Emergency Surgery, Department of General Surgery, The First Affiliated Hospital of Bengbu Medical College, Bnegbu, China
    Liang Pengzhen, Department of Emergency Surgery, Department of Emergency Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui Province, China
    Xu Aman, Department of Gastrointestinal Surgery, First Affiliated Hospital of Anhui Medical University, Hefei, 230022, Anhui Province, China
    Wang Zhenjie, Department of Emergency Surgery, Department of Emergency Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui Province, China

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