AUTHOR=Stutz Claudia , Alcantara Daniel Maximo Correa , Santos Camila Maria dos , Torres Jaire Marinho , Rodrigues Rudielle , Marcon Glaucia Elisete Barbosa , Ferreira Eduardo de Castro , Sarti Elaine Cristina Fernandes Baez , Oliveira Thiago Fernandes de , Mendes Flavia Maria Lins , Lemos Everton Ferreira , Demarchi Luiz Henrique Ferraz , Lichs Gislene Garcia de Castro , Zardin Marina Castilhos Souza Umaki , Gonçalves Crhistinne Cavalheiro Maymone , Guilhermino Jislaine de Fátima , Perdomo Renata Trentin , Fernandez Zoraida del Carmen TITLE=Seroprevalence of antibodies against SARS-CoV-2 in the school community in Campo Grande, state of Mato Grosso do Sul, Brazil, October 2021–November 2022 JOURNAL=Frontiers in Immunology VOLUME=15 YEAR=2024 URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2024.1354786 DOI=10.3389/fimmu.2024.1354786 ISSN=1664-3224 ABSTRACT=Introduction

With the reopening of schools during the coronavirus disease 2019 (COVID-19) pandemic, it was imperative to understand the role of students and education professionals in the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this paper, we determined the seroprevalence of the SARS-CoV-2 anti-nucleocapsid antibodies in the school community in Campo Grande, the capital and most populous city of the state of Mato Grosso do Sul (Brazil) and evaluated its association with sex, school level, and school type.

Materials and methods

The survey was carried out in 20 public and private schools in the urban region of Campo Grande using the TR DPP® COVID-19 immunoglobulin M/immunoglobulin G (IgM/IgG) kit from the Immunobiological Technology Institute (Bio-Manguinhos, Rio de Janeiro, Brazil). Testing was carried out in three periods: from October to December 2021; from March to July 2022; and from August to November 2022. The participants were students aged 6–17 years enrolled in primary or secondary schools and professionals of different ages and roles.

Results

During the first testing period, 162 participants were seropositive for the IgM and/or IgG anti-nucleocapsid SARS-CoV-2 antibodies, with an estimated seroprevalence of 19.6% using Bayesian multilevel regression. In the second period, 251 participants were seropositive (estimated seroprevalence, 34.6%), while in the third period, 393 participants were seroconverted (estimated seroprevalence, 56.7%). In 2022, there was an increase in the seroconversion rate compared to that in 2021. The most frequently described acute manifestations in the three periods were fever, headache, sore throat, and runny nose. In terms of the demographic profile, there was no predominance of seropositivity between the sexes, although women represented approximately 70% of the study population. There were also no differences between students and school staff.

Discussion

The results made it possible to evaluate the extent of SARS-CoV-2 transmission in the school community through immunity developed against the virus, in addition to providing information about COVID-19 symptoms in children, adolescents, and adults.