AUTHOR=Tan Yunhao , Mosallanejad Kenta , Zhang Qingxiu , O’Brien Stephen , Clements Meghan , Perper Stuart , Wilson Sarah , Chaulagain Sudiksha , Wang Jing , Abdalla Mary , Al-Saidi Helen , Butt Danyal , Clabbers Anca , Ofori Kwasi , Dillon Beth , Harvey Bohdan , Memmott John , Negron Christopher , Winarta David , Tan Catherine , Biswas Amlan , Dong Feng , Morales-Tirado Vanessa , Lu Xiaoqing , Singh Gurminder , White Michael , Ashley Shanna , Knight Heather , Westmoreland Susan , Phillips Lucy , Carr Tracy , Reinke-Breen Lauren , Singh Rajeeva , Xu Jianwen , Wu Kan , Rinaldi Lisa , Stoll Brian , He Yupeng David , Hazelwood Lisa , Karman Jozsef , McCluskey Andrew , Stine William , Correia Ivan , Gauld Stephen , Levesque Marc C. , Veldman Geertruida , Hubeau Cedric , Radstake Timothy , Sadhukhan Ramkrishna , Fiebiger Edda TITLE=IL11-mediated stromal cell activation may not be the master regulator of pro-fibrotic signaling downstream of TGFβ JOURNAL=Frontiers in Immunology VOLUME=15 YEAR=2024 URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2024.1293883 DOI=10.3389/fimmu.2024.1293883 ISSN=1664-3224 ABSTRACT=

Fibrotic diseases, such as idiopathic pulmonary fibrosis (IPF) and systemic scleroderma (SSc), are commonly associated with high morbidity and mortality, thereby representing a significant unmet medical need. Interleukin 11 (IL11)-mediated cell activation has been identified as a central mechanism for promoting fibrosis downstream of TGFβ. IL11 signaling has recently been reported to promote fibroblast-to-myofibroblast transition, thus leading to various pro-fibrotic phenotypic changes. We confirmed increased mRNA expression of IL11 and IL11Rα in fibrotic diseases by OMICs approaches and in situ hybridization. However, the vital role of IL11 as a driver for fibrosis was not recapitulated. While induction of IL11 secretion was observed downstream of TGFβ signaling in human lung fibroblasts and epithelial cells, the cellular responses induced by IL11 was quantitatively and qualitatively inferior to that of TGFβ at the transcriptional and translational levels. IL11 blocking antibodies inhibited IL11Rα-proximal STAT3 activation but failed to block TGFβ-induced profibrotic signals. In summary, our results challenge the concept of IL11 blockade as a strategy for providing transformative treatment for fibrosis.