AUTHOR=Li Xiaohan , Chen Zhang , Ye Weicong , Yu Jizhang , Zhang Xi , Li Yuan , Niu Yuqing , Ran Shuan , Wang Song , Luo Zilong , Zhao Jiulu , Hao Yanglin , Zong Junjie , Xia Chengkun , Xia Jiahong , Wu Jie TITLE=High-throughput CRISPR technology: a novel horizon for solid organ transplantation JOURNAL=Frontiers in Immunology VOLUME=14 YEAR=2024 URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2023.1295523 DOI=10.3389/fimmu.2023.1295523 ISSN=1664-3224 ABSTRACT=

Organ transplantation is the gold standard therapy for end-stage organ failure. However, the shortage of available grafts and long-term graft dysfunction remain the primary barriers to organ transplantation. Exploring approaches to solve these issues is urgent, and CRISPR/Cas9-based transcriptome editing provides one potential solution. Furthermore, combining CRISPR/Cas9-based gene editing with an ex vivo organ perfusion system would enable pre-implantation transcriptome editing of grafts. How to determine effective intervention targets becomes a new problem. Fortunately, the advent of high-throughput CRISPR screening has dramatically accelerated the effective targets. This review summarizes the current advancements, utilization, and workflow of CRISPR screening in various immune and non-immune cells. It also discusses the ongoing applications of CRISPR/Cas-based gene editing in transplantation and the prospective applications of CRISPR screening in solid organ transplantation.