AUTHOR=Bazzi Wael , Monticelli Sara , Delaporte Claude , Riet Céline , Giangrande Angela , Cattenoz Pierre B. 

TITLE=Gcm counteracts Toll-induced inflammation and impacts hemocyte number through cholinergic signaling

JOURNAL=Frontiers in Immunology

VOLUME=Volume 14 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2023.1293766

DOI=10.3389/fimmu.2023.1293766

ISSN=1664-3224

ABSTRACT=Hemocytes, the myeloid-like immune cells of Drosophila, fulfil a variety of functions that are not completely understood, ranging from phagocytosis to transduction of inflammatory signals. We here show that downregulating the hemocyte-specific Glide/Gcm transcription factor enhances the inflammatory response to the constitutive activation of the Toll pathway. This correlates with lower levels of glutathione S transferase, suggesting an implication of Glide/Gcm in ROS signaling and calling for a widespread anti-inflammatory potential of Glide/Gcm. In addition, our data We showreveal the expression of neurotransmitter acetylcholine receptors in hemocytes and that Toll activation affects their expressions, disclosing a novel aspect of the inflammatory response mediated by neurotransmitters. Finally, we provide evidence for acetylcholine receptor nAchRalpha6 regulating hemocyte proliferation in a cell autonomous fashion and for Finally, we provide evidence for acetylcholine receptor nAchRalpha6 regulating hemocyte proliferationnon-cell autonomous cholinergic signaling regulating the number of hemocytes. Altogether, this study provides new insights on the molecular pathways involved in the inflammatory response.