AUTHOR=Lu Yujie , Gu Dingyi , Zhao Chenyi , Sun Ying , Li Wenjing , He Lulu , Wang Xiaoyan , Kou Zhongyang , Su Jiang , Guo Feng 

TITLE=Genomic landscape and expression profile of consensus molecular subtype four of colorectal cancer

JOURNAL=Frontiers in Immunology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2023.1160052

DOI=10.3389/fimmu.2023.1160052

ISSN=1664-3224

ABSTRACT=<sec><title>Background</title><p>Compared to other subtypes, the CMS4 subtype is associated with lacking of effective treatments and poorer survival rates.</p></sec><sec><title>Methods</title><p>A total of 24 patients with CRC were included in this study. DNA and RNA sequencing were performed to acquire somatic mutations and gene expression, respectively. MATH was used to quantify intratumoral heterogeneity. PPI and survival analyses were performed to identify hub DEGs. Reactome and KEGG analyses were performed to analyze the pathways of mutated or DEGs. Single-sample gene set enrichment analysis and Xcell were used to categorize the infiltration of immune cells.</p></sec><sec><title>Results</title><p>The CMS4 patients had a poorer PFS than CMS2/3. <italic>CTNNB1</italic> and <italic>CCNE1</italic> were common mutated genes in the CMS4 subtype, which were enriched in Wnt and cell cycle signaling pathways, respectively. The MATH score of CMS4 subtype was lower. <italic>SLC17A6</italic> was a hub DEG. M2 macrophages were more infiltrated in the tumor microenvironment of CMS4 subtype. The CMS4 subtype tended to have an immunosuppressive microenvironment.</p></sec><sec><title>Conclusion</title><p>This study suggested new perspectives for exploring therapeutic strategies for the CMS4 subtype CRC.</p></sec>