AUTHOR=King Hannah A. D. , Dussupt Vincent , Mendez-Rivera Letzibeth , Slike Bonnie M. , Tran Ursula , Jackson Nathan D. , Barkei Erica , Zemil Michelle , Tourtellott-Fogt Emily , Kuklis Caitlin H. , Soman Sandrine , Ahmed Aslaa , Porto Maciel , Kitajewski Christopher , Spence Brittany , Benetiene Dalia , Wieczorek Lindsay , Kar Swagata , Gromowski Gregory , Polonis Victoria R. , Krebs Shelly J. , Modjarrad Kayvon , Bolton Diane L. TITLE=Convalescent human IgG, but not IgM, from COVID-19 survivors confers dose-dependent protection against SARS-CoV-2 replication and disease in hamsters JOURNAL=Frontiers in Immunology VOLUME=14 YEAR=2023 URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2023.1138629 DOI=10.3389/fimmu.2023.1138629 ISSN=1664-3224 ABSTRACT=Introduction

Antibody therapeutic strategies have served an important role during the COVID-19 pandemic, even as their effectiveness has waned with the emergence of escape variants. Here we sought to determine the concentration of convalescent immunoglobulin required to protect against disease from SARS-CoV-2 in a Syrian golden hamster model.

Methods

Total IgG and IgM were isolated from plasma of SARS-CoV-2 convalescent donors. Dose titrations of IgG and IgM were infused into hamsters 1 day prior to challenge with SARS-CoV-2 Wuhan-1.

Results

The IgM preparation was found to have ~25-fold greater neutralization potency than IgG. IgG infusion protected hamsters from disease in a dose-dependent manner, with detectable serum neutralizing titers correlating with protection. Despite a higher in vitro neutralizing potency, IgM failed to protect against disease when transferred into hamsters.

Discussion

This study adds to the growing body of literature that demonstrates neutralizing IgG antibodies are important for protection from SARS-CoV-2 disease, and confirms that polyclonal IgG in sera can be an effective preventative strategy if the neutralizing titers are sufficiently high. In the context of new variants, against which existing vaccines or monoclonal antibodies have reduced efficacy, sera from individuals who have recovered from infection with the emerging variant may potentially remain an efficacious tool.