AUTHOR=Velásquez Zahady D. , Peixoto Raquel , Gärtner Ulrich , Hermosilla Carlos , Taubert Anja , Conejeros Iván 

TITLE=Dynamics of cell cycle proteins involved in Toxoplasma gondii-induced bovine NET formation

JOURNAL=Frontiers in Immunology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2023.1125667

DOI=10.3389/fimmu.2023.1125667

ISSN=1664-3224

ABSTRACT=<p>Neutrophil extracellular traps (NET) formation is one important host innate defense mechanism elicited by polymorphonuclear neutrophils (PMN). NETs are composed by chromatin and proteins with microbicidal and signaling activity. So far, there is one report on <italic>Toxoplasma gondii-</italic>triggered NETs in cattle, however, exact mechanisms, including signalling pathways and dynamics governing this reaction remain largely unknown. Recently, involvement of cell cycle proteins was demonstrated for phorbol myristate acetate (PMA)-triggered human PMN-derived NETs. Here, we studied the involvement of cell cycle proteins in <italic>T. gondii</italic>-induced NETs in exposed bovine PMN. Through confocal and transmission electron microscopy we discovered that Ki-67 and lamin B1 signals are upregulated and relocated during <italic>T. gondii</italic>-induced NETosis. Nuclear membrane disruption was also observed as a hallmark of NET formation in bovine PMN confronted with viable <italic>T. gondii</italic> tachyzoites, mimicking some steps of mitosis. However, we did not observe centrosome duplication as previously described for human PMN-derived NET formation stimulated with PMA.</p>